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Computer simulation of weak affinity chromatography

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  • M. Wikstrom
  • S. Ohlson
Affinity chromatography is a selective purification technique in which the specific interactions between the bound ligand and the solutes (ligates) in the mobile phase are of high affinity [the equilibrium constant (K(L)) is usually between 10 and 10 l mol]. By utilizing weaker affinities (K(L) <10 l mol), fast and dynamic separations with high performance can be achieved exploiting the specificity introduced by the affinity ligand. As weak affinity chromatography (WAC) is a recent technique and the number of applications is limited, computer simulation of established mathematical models for chromatography was applied for the purpose of studying the basics of WAC and its applicability. The antigen-antibody system was selected owing to its general nature and permitted direct comparisons with experimental data. Important parameters such as particle size of support, kinetics, ligate concentration, bound ligand concentration and peak capacity were evaluated to determine their significance in WAC. A fit of the model to the experimental data from the antigen-antibody system generated information on the kinetics of the interaction and the number of active accessible sites.
TidsskriftJournal of Chromatography A
Udgave nummer1-2
Sider (fra-til)83-92
Antal sider10
StatusUdgivet - 24 apr. 1992
Eksternt udgivetJa

ID: 47970064