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Construction and Use of Flow Cytometry Optimized Plasmid-Sensor Strains: Genomes in Flux

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskning

Determining the stability of plasmids in bacterial populations is traditionally performed by isolating a large number of clones followed by screening for the presence of plasmids by replica transfer to plasmid-selective agar plates. This is often a laborious task, especially when the intrinsic stability of the plasmid is high. The method presented here relies on a phenotypic (green fluorescence protein) marker, which is switched on if the host bacteria loses the residing plasmid. The incorporation of flow cytometry for single-cell detection and discrimination between plasmid-free and plasmid-harboring cells in a bacterial population facilitates a very high throughput of cells and thus provides excellent sensitivity and statistics toward detecting even very low levels of plasmid instability.
OriginalsprogEngelsk
TitelHorizontal Gene Transfer
RedaktørerMaria Boekels Gogarten, Johann Peter Gogarten, Lorraine C. Olendzenski
Vol/bind532
ForlagHumana Press
Publikationsdato2009
Sider257-268
ISBN (Trykt)978-1-60327-852-2
ISBN (Elektronisk)978-1-60327-853-9
DOI
StatusUdgivet - 2009
NavnMethods in Molecular Biology
ISSN1064-3745

ID: 11663620