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Contraction-induced lipolysis is not impaired by inhibition of hormone-sensitive lipase in skeletal muscle

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In skeletal muscle hormone-sensitive lipase (HSL) has long been accepted to be the principal enzyme responsible for lipolysis of intramyocellular triacylglycerol (IMTG) during contractions. However, this notion is based on in vitro lipase activity data, which may not reflect the in vivo lipolytic activity. We investigated lipolysis of IMTG in soleus muscles electrically-stimulated to contract ex vivo during acute pharmacological inhibition of HSL in rat muscles and in muscles from HSL-KO mice. Measurements of IMTG are complicated by the presence of adipocytes located between the muscle fibers. To circumvent the problem with this contamination we analyzed intramyocellular lipid droplet content histochemically. At maximal inhibition of HSL in rat muscles, contraction-induced breakdown of IMTG was identical to that seen in control muscles (p
OriginalsprogEngelsk
TidsskriftJournal of Physiology
Vol/bind591
Udgave nummer20
Sider (fra-til)5141-5155
Antal sider15
ISSN0022-3751
DOI
StatusUdgivet - 2013

Bibliografisk note

CURIS 2013 NEXS 175

ID: 49037799