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CPT1a-Dependent Long-Chain Fatty Acid Oxidation Contributes to Maintaining Glucagon Secretion from Pancreatic Islets

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Linford J B Briant
  • Michael S Dodd
  • Margarita V Chibalina
  • Nils J G Rorsman
  • Paul R V Johnson
  • Peter Carmeliet
  • Patrik Rorsman
  • Knudsen, Jakob Grunnet

Glucagon, the principal hyperglycemic hormone, is secreted from pancreatic islet α cells as part of the counter-regulatory response to hypoglycemia. Hence, secretory output from α cells is under high demand in conditions of low glucose supply. Many tissues oxidize fat as an alternate energy substrate. Here, we show that glucagon secretion in low glucose conditions is maintained by fatty acid metabolism in both mouse and human islets, and that inhibiting this metabolic pathway profoundly decreases glucagon output by depolarizing α cell membrane potential and decreasing action potential amplitude. We demonstrate, by using experimental and computational approaches, that this is not mediated by the KATP channel, but instead due to reduced operation of the Na+-K+ pump. These data suggest that counter-regulatory secretion of glucagon is driven by fatty acid metabolism, and that the Na+-K+ pump is an important ATP-dependent regulator of α cell function.

OriginalsprogEngelsk
TidsskriftCell Reports
Vol/bind23
Udgave nummer11
Sider (fra-til)3300-3311
Antal sider12
ISSN2211-1247
DOI
StatusUdgivet - 2018
Eksternt udgivetJa

Bibliografisk note

Copyright © 2018 The Author(s). Published by Elsevier Inc. All rights reserved.

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