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CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling

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CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling. / Abdallah, Basem M.; Figeac, Florence; Larsen, Kenneth H.; Ditzel, Nicholas; Keshari, Pankaj; Isa, Adiba; Jafari Kermani, Abbas; Andersen, Thomas L; Delaisse, Jean-Marie; Goshima, Yoshio; Ohshima, Toshio; Kassem, Moustapha.

I: Journal of Bone and Mineral Research, Bind 32, Nr. 5, 05.2017, s. 913-926.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Abdallah, BM, Figeac, F, Larsen, KH, Ditzel, N, Keshari, P, Isa, A, Jafari Kermani, A, Andersen, TL, Delaisse, J-M, Goshima, Y, Ohshima, T & Kassem, M 2017, 'CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling', Journal of Bone and Mineral Research, bind 32, nr. 5, s. 913-926. https://doi.org/10.1002/jbmr.3069

APA

Abdallah, B. M., Figeac, F., Larsen, K. H., Ditzel, N., Keshari, P., Isa, A., ... Kassem, M. (2017). CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling. Journal of Bone and Mineral Research, 32(5), 913-926. https://doi.org/10.1002/jbmr.3069

Vancouver

Abdallah BM, Figeac F, Larsen KH, Ditzel N, Keshari P, Isa A o.a. CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling. Journal of Bone and Mineral Research. 2017 maj;32(5):913-926. https://doi.org/10.1002/jbmr.3069

Author

Abdallah, Basem M. ; Figeac, Florence ; Larsen, Kenneth H. ; Ditzel, Nicholas ; Keshari, Pankaj ; Isa, Adiba ; Jafari Kermani, Abbas ; Andersen, Thomas L ; Delaisse, Jean-Marie ; Goshima, Yoshio ; Ohshima, Toshio ; Kassem, Moustapha. / CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling. I: Journal of Bone and Mineral Research. 2017 ; Bind 32, Nr. 5. s. 913-926.

Bibtex

@article{b54b6292dcea40cab8b329257a7ee402,
title = "CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling",
abstract = "We identified the neuroprotein collapsing response mediator protein-4 (CRMP4) as a noncanonical osteogenic factor that regulates the differentiation of mouse bone marrow skeletal stem cells (bone marrow stromal stem cells [mBMSCs]) into osteoblastic cells. CRMP4 is the only member of the CRMP1-CRMP5 family to be expressed by mBMSCs and in osteoprogenitors of both adult mouse and human bones. In vitro gain-of-function and loss-of-function of CRMP4 in murine stromal cells revealed its inhibitory effect on osteoblast differentiation. In addition, Crmp4-deficient mice (Crmp4(-/-) ) displayed a 40{\%} increase in bone mass, increased mineral apposition rate, and bone formation rate, compared to wild-type controls. Increased bone mass in Crmp4(-/-) mice was associated with enhanced BMP2 signaling and BMP2-induced osteoblast differentiation in Crmp4(-/-) osteoblasts (OBs). Furthermore, Crmp4(-/-) OBs exhibited enhanced activation of RhoA/focal adhesion kinase (FAK) signaling that led to cytoskeletal changes with increased cell spreading. In addition, Crmp4(-/-) OBs exhibited increased cell proliferation that was mediated via inhibiting cyclin-dependent kinase inhibitor 1B, p27(Kip1) and upregulating cyclin D1 expression which are targets of RhoA signaling pathway. Our findings identify CRMP4 as a novel negative regulator of osteoblast differentiation. {\circledC} 2017 American Society for Bone and Mineral Research.[on SciFinder (R)]",
keywords = "bone remodeling, crmp4, dpysl3, osteoblast, osteoporosis",
author = "Abdallah, {Basem M.} and Florence Figeac and Larsen, {Kenneth H.} and Nicholas Ditzel and Pankaj Keshari and Adiba Isa and {Jafari Kermani}, Abbas and Andersen, {Thomas L} and Jean-Marie Delaisse and Yoshio Goshima and Toshio Ohshima and Moustapha Kassem",
note = "M1 - Copyright (C) 2017 U.S. National Library of Medicine. MEDLINE AN 2018089741(Journal; Article; (JOURNAL ARTICLE))",
year = "2017",
month = "5",
doi = "10.1002/jbmr.3069",
language = "English",
volume = "32",
pages = "913--926",
journal = "Journal of Bone and Mineral Research",
issn = "0884-0431",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - CRMP4 Inhibits Bone Formation by Negatively Regulating BMP and RhoA Signaling

AU - Abdallah, Basem M.

AU - Figeac, Florence

AU - Larsen, Kenneth H.

AU - Ditzel, Nicholas

AU - Keshari, Pankaj

AU - Isa, Adiba

AU - Jafari Kermani, Abbas

AU - Andersen, Thomas L

AU - Delaisse, Jean-Marie

AU - Goshima, Yoshio

AU - Ohshima, Toshio

AU - Kassem, Moustapha

N1 - M1 - Copyright (C) 2017 U.S. National Library of Medicine. MEDLINE AN 2018089741(Journal; Article; (JOURNAL ARTICLE))

PY - 2017/5

Y1 - 2017/5

N2 - We identified the neuroprotein collapsing response mediator protein-4 (CRMP4) as a noncanonical osteogenic factor that regulates the differentiation of mouse bone marrow skeletal stem cells (bone marrow stromal stem cells [mBMSCs]) into osteoblastic cells. CRMP4 is the only member of the CRMP1-CRMP5 family to be expressed by mBMSCs and in osteoprogenitors of both adult mouse and human bones. In vitro gain-of-function and loss-of-function of CRMP4 in murine stromal cells revealed its inhibitory effect on osteoblast differentiation. In addition, Crmp4-deficient mice (Crmp4(-/-) ) displayed a 40% increase in bone mass, increased mineral apposition rate, and bone formation rate, compared to wild-type controls. Increased bone mass in Crmp4(-/-) mice was associated with enhanced BMP2 signaling and BMP2-induced osteoblast differentiation in Crmp4(-/-) osteoblasts (OBs). Furthermore, Crmp4(-/-) OBs exhibited enhanced activation of RhoA/focal adhesion kinase (FAK) signaling that led to cytoskeletal changes with increased cell spreading. In addition, Crmp4(-/-) OBs exhibited increased cell proliferation that was mediated via inhibiting cyclin-dependent kinase inhibitor 1B, p27(Kip1) and upregulating cyclin D1 expression which are targets of RhoA signaling pathway. Our findings identify CRMP4 as a novel negative regulator of osteoblast differentiation. © 2017 American Society for Bone and Mineral Research.[on SciFinder (R)]

AB - We identified the neuroprotein collapsing response mediator protein-4 (CRMP4) as a noncanonical osteogenic factor that regulates the differentiation of mouse bone marrow skeletal stem cells (bone marrow stromal stem cells [mBMSCs]) into osteoblastic cells. CRMP4 is the only member of the CRMP1-CRMP5 family to be expressed by mBMSCs and in osteoprogenitors of both adult mouse and human bones. In vitro gain-of-function and loss-of-function of CRMP4 in murine stromal cells revealed its inhibitory effect on osteoblast differentiation. In addition, Crmp4-deficient mice (Crmp4(-/-) ) displayed a 40% increase in bone mass, increased mineral apposition rate, and bone formation rate, compared to wild-type controls. Increased bone mass in Crmp4(-/-) mice was associated with enhanced BMP2 signaling and BMP2-induced osteoblast differentiation in Crmp4(-/-) osteoblasts (OBs). Furthermore, Crmp4(-/-) OBs exhibited enhanced activation of RhoA/focal adhesion kinase (FAK) signaling that led to cytoskeletal changes with increased cell spreading. In addition, Crmp4(-/-) OBs exhibited increased cell proliferation that was mediated via inhibiting cyclin-dependent kinase inhibitor 1B, p27(Kip1) and upregulating cyclin D1 expression which are targets of RhoA signaling pathway. Our findings identify CRMP4 as a novel negative regulator of osteoblast differentiation. © 2017 American Society for Bone and Mineral Research.[on SciFinder (R)]

KW - bone remodeling

KW - crmp4

KW - dpysl3

KW - osteoblast

KW - osteoporosis

U2 - 10.1002/jbmr.3069

DO - 10.1002/jbmr.3069

M3 - Journal article

C2 - 28019696

VL - 32

SP - 913

EP - 926

JO - Journal of Bone and Mineral Research

JF - Journal of Bone and Mineral Research

SN - 0884-0431

IS - 5

ER -

ID: 173291061