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CtBP1-Mediated Membrane Fission Contributes to Effective Recycling of Synaptic Vesicles

Publikation: Bidrag til tidsskriftTidsskriftartikel

  • Daniela Ivanova
  • Imig, Cordelia
  • Marcial Camacho
  • Annika Reinhold
  • Debarpan Guhathakurta
  • Carolina Montenegro-Venegas
  • Michael A Cousin
  • Eckart D Gundelfinger
  • Christian Rosenmund
  • Benjamin Cooper
  • Anna Fejtova

Compensatory endocytosis of released synaptic vesicles (SVs) relies on coordinated signaling at the lipid-protein interface. Here, we address the synaptic function of C-terminal binding protein 1 (CtBP1), a ubiquitous regulator of gene expression and membrane trafficking in cultured hippocampal neurons. In the absence of CtBP1, synapses form in greater density and show changes in SV distribution and size. The increased basal neurotransmission and enhanced synaptic depression could be attributed to a higher vesicular release probability and a smaller fraction of release-competent SVs, respectively. Rescue experiments with specifically targeted constructs indicate that, while synaptogenesis and release probability are controlled by nuclear CtBP1, the efficient recycling of SVs relies on its synaptic expression. The ability of presynaptic CtBP1 to facilitate compensatory endocytosis depends on its membrane-fission activity and the activation of the lipid-metabolizing enzyme PLD1. Thus, CtBP1 regulates SV recycling by promoting a permissive lipid environment for compensatory endocytosis.

OriginalsprogEngelsk
TidsskriftCell Reports
Vol/bind30
Udgave nummer7
Sider (fra-til)2444-2459.e7
ISSN2211-1247
DOI
StatusUdgivet - 18 feb. 2020
Eksternt udgivetJa

ID: 237696785