Forskning ved Københavns Universitet - Københavns Universitet


Derivatives of amphotericin inhibit infection with human immunodeficiency virus in vitro by different modes of action.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

  • J E Hansen
  • N M Witzke
  • C Nielsen
  • Lars Reinhardt Mathiesen
  • L S Teglbjaerg
  • C M Nielsen
  • Jens Ole Nielsen
Three water-soluble derivatives of amphotericin B were tested for inhibition of HIV infection in vitro. The compounds amphotericin B methyl ester (AME) and N-(N'-(2-(4'-methylmorpholinio)ethyl)N"-cyclohexyl guanyl) amphotericin B methyl ester (MCG) inhibited HIV infection by 50% at 1 microgram/ml; N-(N'-(3-dimethylaminopropyl)N"-ethyl guanyl) amphotericin B (DAPEG) did so at 5-11 micrograms/ml. While the virus-inhibitory effect of AME was due to an interaction with target lymphocytes, the effect of MCG was due to a direct anti-viral action. AME increased the potential of infected cells to fuse with uninfected cells, but MCG had no significant effect on cell fusion. All compounds had a lower cellular toxicity than amphotericin B and were not toxic at concentrations below 20 micrograms/ml.
TidsskriftAntiviral Research
Udgave nummer3
Sider (fra-til)149-159
Antal sider11
StatusUdgivet - 1990

ID: 34125891