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Determination of psilocybin in Psilocybe semilanceata by capillary zone electrophoresis

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A capillary zone electrophoretic (CZE) method was developed for the rapid determination of psilocybin in Psilocybe semilanceata. Following a simple two step extraction with 3.0+2.0 ml methanol, the hallucinogenic compound was effectively separated from matrix components by CZE utilizing a 10 mM borate-phosphate running buffer adjusted to pH 11.5. The identity of psilocybin was confirmed by migration time information and by W spectra, while quantitation was accomplished utilizing barbital as internal standard. The calibration curve for psilocybin was linear within 0.01-1 mg/ml, while intra-day and inter-day variations of quantitative data were 0.5 and 2.5% R.S.D., respectively. In addition to psilocybin, the method was also suitable for the determination of the structurally related compound baeocystin.

OriginalsprogEngelsk
TidsskriftJournal of Chromatography B: Biomedical Applications
Vol/bind694
Udgave nummer2
Sider (fra-til)375-381
Antal sider7
ISSN1572-6495
DOI
StatusUdgivet - 4 jul. 1997

ID: 231655754