Forskning ved Københavns Universitet - Københavns Universitet

Forside

Developmental kinetics of the first cell cycles of bovine in vitro PRODUCED EMBRYOS IN RELATION TO THEIR IN VITRO VIABILITY AND SEX

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Holm, Peter
  • N.N Shukri
  • Gabor Vajta
  • C Bendixen
  • Henrik Callesen
The development of bovine IVP-embryos was observed in a time-lapse culture system to determine cell cycle lengths of 1) embryos that developed into compact morulae (CM) or blastocysts (BL) within 174 h after insemination (viable), 2) embryos that arrested during earlier stages (nonviable) and 3) male and female embryos. In 4 replicates, inseminated oocytes were cultured on a microscope stage in 3 to 4 groups on a granulosa cell monolayer in supplemented TCM 199. Images were sequentially recorded and stored at 30-min intervals. All embryos that could be identified throughout the culture period were included (n=392), and the times of cleavage events noted. After culture, 100 CM or BL were randomly selected for sexing by PCR. BL developed equally well in the time-lapse and control culture systems (36 vs 38. The respective lengths of the first 4 cell cycles of viable embryos were 32.0 + 3.9, g.8 + 1.6, 10.8 + 4.7 and 47.7 + 11.8 h. The subsequent intervals between the 9- to 16-cell, early morula, CM and BL stages lasted 16.2 to 18.2 h. Blastomeres of 2-, 4- and 8-cell embryos cleaved asynchronously with
OriginalsprogEngelsk
TidsskriftTheriogenology
Vol/bind98
Sider (fra-til)1285-1299
Antal sider15
ISSN0093-691X
StatusUdgivet - 1998

    Forskningsområder

  • bovine,cell cycle length,embryo kinetics,in vitro development,time-lapse recording

ID: 141569145