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Diagnostic double guarded low-volume uterine lavage in mares

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningfagfællebedømt

Standard

Diagnostic double guarded low-volume uterine lavage in mares. / Christoffersen, Mette; Brandis, Louise; Samuelsson, Julia; Bojesen, Anders Miki; Troedsson, M.H.T.; Petersen, M. R.

I: Clinical Theriogenology, Bind 6, Nr. 3, 07.08.2014, s. 393.

Publikation: Bidrag til tidsskriftKonferenceabstrakt i tidsskriftForskningfagfællebedømt

Harvard

Christoffersen, M, Brandis, L, Samuelsson, J, Bojesen, AM, Troedsson, MHT & Petersen, MR 2014, 'Diagnostic double guarded low-volume uterine lavage in mares', Clinical Theriogenology, bind 6, nr. 3, s. 393.

APA

Christoffersen, M., Brandis, L., Samuelsson, J., Bojesen, A. M., Troedsson, M. H. T., & Petersen, M. R. (2014). Diagnostic double guarded low-volume uterine lavage in mares. Clinical Theriogenology, 6(3), 393.

Vancouver

Christoffersen M, Brandis L, Samuelsson J, Bojesen AM, Troedsson MHT, Petersen MR. Diagnostic double guarded low-volume uterine lavage in mares. Clinical Theriogenology. 2014 aug 7;6(3):393.

Author

Christoffersen, Mette ; Brandis, Louise ; Samuelsson, Julia ; Bojesen, Anders Miki ; Troedsson, M.H.T. ; Petersen, M. R. / Diagnostic double guarded low-volume uterine lavage in mares. I: Clinical Theriogenology. 2014 ; Bind 6, Nr. 3. s. 393.

Bibtex

@article{4fcf2f0184434df1820ac501f7869bde,
title = "Diagnostic double guarded low-volume uterine lavage in mares",
abstract = "Endometritis constitutes a major problem in the management of broodmares; hence diagnostic tests with a high sensitivity and specificity are desired. We hypothesize that a double guarded uterine flush technique for bacterial culture and cytology is comparable to standard diagnostic tests, the endometrial biopsy and double guarded swab. Endometrial biopsies (n=199), swabs (n=199) and double guarded lavage samples (n=199) were obtained from 34 mares at six different time points in four estrous cycles, and were evaluated cytologically and bacteriologically. Endometrial biopsies from the first cycle (n=34) were examined for the presence of polymorphonuclear neutrophils (PMNs) in the endometrial epithelium and was used as a gold standard for calculation of diagnostic sensitivity. E. coli was most frequently isolated (lavage: 31{\%}, swab: 21{\%}, biopsy: 12{\%}) followed by β-hemolytic streptococci (lavage: 11{\%}, swab: 8{\%}, biopsy: 7{\%}) (positive bacterial growth > 4 Colony forming units (CFU)). Positive cytology was less likely to occur when E. coli was isolated from the diagnostic tests compared to the growth of β-hemolytic streptococci. Isolation of pathogens from uterine samples was highly associated with the presence of PMNs on histology (p=0.003). Using the presence of PMNs in the endometrial tissue as the gold standard for diagnosing endometritis, the sensitivity of double guarded lavage culture was 0.75, and 0.33 and 0.5 for the swab and biopsy, respectively. In conclusion, the double guarded lavage technique was an accurate method for diagnosing mares with endometritis and the risk of false positive samples is considered to be minimal compared to other flushing techniques described. Key words: Endometritis, double guarded lavage, diagnostic test, E. coli, β-hemolytic streptococci",
author = "Mette Christoffersen and Louise Brandis and Julia Samuelsson and Bojesen, {Anders Miki} and M.H.T. Troedsson and Petersen, {M. R.}",
year = "2014",
month = "8",
day = "7",
language = "English",
volume = "6",
pages = "393",
journal = "Clinical Theriogenology",
issn = "2154-3968",
number = "3",
note = "null ; Conference date: 06-08-2014 Through 09-08-2014",

}

RIS

TY - ABST

T1 - Diagnostic double guarded low-volume uterine lavage in mares

AU - Christoffersen, Mette

AU - Brandis, Louise

AU - Samuelsson, Julia

AU - Bojesen, Anders Miki

AU - Troedsson, M.H.T.

AU - Petersen, M. R.

PY - 2014/8/7

Y1 - 2014/8/7

N2 - Endometritis constitutes a major problem in the management of broodmares; hence diagnostic tests with a high sensitivity and specificity are desired. We hypothesize that a double guarded uterine flush technique for bacterial culture and cytology is comparable to standard diagnostic tests, the endometrial biopsy and double guarded swab. Endometrial biopsies (n=199), swabs (n=199) and double guarded lavage samples (n=199) were obtained from 34 mares at six different time points in four estrous cycles, and were evaluated cytologically and bacteriologically. Endometrial biopsies from the first cycle (n=34) were examined for the presence of polymorphonuclear neutrophils (PMNs) in the endometrial epithelium and was used as a gold standard for calculation of diagnostic sensitivity. E. coli was most frequently isolated (lavage: 31%, swab: 21%, biopsy: 12%) followed by β-hemolytic streptococci (lavage: 11%, swab: 8%, biopsy: 7%) (positive bacterial growth > 4 Colony forming units (CFU)). Positive cytology was less likely to occur when E. coli was isolated from the diagnostic tests compared to the growth of β-hemolytic streptococci. Isolation of pathogens from uterine samples was highly associated with the presence of PMNs on histology (p=0.003). Using the presence of PMNs in the endometrial tissue as the gold standard for diagnosing endometritis, the sensitivity of double guarded lavage culture was 0.75, and 0.33 and 0.5 for the swab and biopsy, respectively. In conclusion, the double guarded lavage technique was an accurate method for diagnosing mares with endometritis and the risk of false positive samples is considered to be minimal compared to other flushing techniques described. Key words: Endometritis, double guarded lavage, diagnostic test, E. coli, β-hemolytic streptococci

AB - Endometritis constitutes a major problem in the management of broodmares; hence diagnostic tests with a high sensitivity and specificity are desired. We hypothesize that a double guarded uterine flush technique for bacterial culture and cytology is comparable to standard diagnostic tests, the endometrial biopsy and double guarded swab. Endometrial biopsies (n=199), swabs (n=199) and double guarded lavage samples (n=199) were obtained from 34 mares at six different time points in four estrous cycles, and were evaluated cytologically and bacteriologically. Endometrial biopsies from the first cycle (n=34) were examined for the presence of polymorphonuclear neutrophils (PMNs) in the endometrial epithelium and was used as a gold standard for calculation of diagnostic sensitivity. E. coli was most frequently isolated (lavage: 31%, swab: 21%, biopsy: 12%) followed by β-hemolytic streptococci (lavage: 11%, swab: 8%, biopsy: 7%) (positive bacterial growth > 4 Colony forming units (CFU)). Positive cytology was less likely to occur when E. coli was isolated from the diagnostic tests compared to the growth of β-hemolytic streptococci. Isolation of pathogens from uterine samples was highly associated with the presence of PMNs on histology (p=0.003). Using the presence of PMNs in the endometrial tissue as the gold standard for diagnosing endometritis, the sensitivity of double guarded lavage culture was 0.75, and 0.33 and 0.5 for the swab and biopsy, respectively. In conclusion, the double guarded lavage technique was an accurate method for diagnosing mares with endometritis and the risk of false positive samples is considered to be minimal compared to other flushing techniques described. Key words: Endometritis, double guarded lavage, diagnostic test, E. coli, β-hemolytic streptococci

M3 - Conference abstract in journal

VL - 6

SP - 393

JO - Clinical Theriogenology

JF - Clinical Theriogenology

SN - 2154-3968

IS - 3

Y2 - 6 August 2014 through 9 August 2014

ER -

ID: 138312836