Forskning ved Københavns Universitet - Københavns Universitet


Differential adaptations of insulin-like growth factor-I, basic fibroblast growth factor, and leukemia inhibitory factor in the plantaris muscle of rats by mechanical overloading: An immunohistochemical study

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  • Kunihiro Sakuma
  • Kimi Watanabe
  • Tsuyoshi Totsuka
  • Isao Uramoto
  • Mamoru Sano
  • Sakamoto, Kei

We investigated changes in several growth factors in the rat plantaris muscle produced by mechanical overloading by ablation of synergists using immunohistochemistry. At 1 and 3 days post surgery, the insulin-like growth factor-I (IGF-I) level was slightly increased in the cytosol and markedly increased in the invading cells of the extracellular space. Thereafter, the IGF-I immunoreactivity evoked by overloading rapidly decreased to the normal level. The level of leukemia inhibitory factor (LIF), which was not shown to change at 1 day post surgery, was increased in the cytosol at 3, 5, 7 and 10 days and at 2 weeks. Basic fibroblast growth factor (bFGF) immunoreactivity did not change during the entire period of overloading (1 day-3 weeks post surgery). These results indicate that the elevations of the levels of IGF-I and LIF show differential time course in the plantaris muscle subjected to functional overload. Furthermore, bFGF appears not to be related to the compensatory hypertrophy produced by overloading.

TidsskriftActa Neuropathologica
Udgave nummer2
Sider (fra-til)123-130
Antal sider8
StatusUdgivet - 1 jan. 1998
Eksternt udgivetJa

ID: 239779635