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Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells

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Standard

Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells. / Dreher, Anita; Rossing, Maria; Kaczkowski, Bogumil; Andersen, Ditte K; Larsen, Therese Juhlin; Christophersen, Mikael Kronborg; Nielsen, Finn Cilius; Norrild, Bodil.

I: Biochemical and Biophysical Research Communications, Bind 412, Nr. 1, 2011, s. 20-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Dreher, A, Rossing, M, Kaczkowski, B, Andersen, DK, Larsen, TJ, Christophersen, MK, Nielsen, FC & Norrild, B 2011, 'Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells', Biochemical and Biophysical Research Communications, bind 412, nr. 1, s. 20-5. https://doi.org/10.1016/j.bbrc.2011.07.011

APA

Dreher, A., Rossing, M., Kaczkowski, B., Andersen, D. K., Larsen, T. J., Christophersen, M. K., ... Norrild, B. (2011). Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells. Biochemical and Biophysical Research Communications, 412(1), 20-5. https://doi.org/10.1016/j.bbrc.2011.07.011

Vancouver

Dreher A, Rossing M, Kaczkowski B, Andersen DK, Larsen TJ, Christophersen MK o.a. Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells. Biochemical and Biophysical Research Communications. 2011;412(1):20-5. https://doi.org/10.1016/j.bbrc.2011.07.011

Author

Dreher, Anita ; Rossing, Maria ; Kaczkowski, Bogumil ; Andersen, Ditte K ; Larsen, Therese Juhlin ; Christophersen, Mikael Kronborg ; Nielsen, Finn Cilius ; Norrild, Bodil. / Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells. I: Biochemical and Biophysical Research Communications. 2011 ; Bind 412, Nr. 1. s. 20-5.

Bibtex

@article{347fdbfdeee84fed98bfb72b58fe164b,
title = "Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells",
abstract = "Human papillomaviruses (HPVs) are highly prevalent giving rise to both benign and malignant lesions why they are classified as high- and low-risk viruses. In this study we selected one low-risk (HPV 11) and two high-risk (HPV 16 and -45) types for genomewide miRNA analysis to investigate possible common and distinct features in the expression profiles. For this purpose we developed a cell culture model system in HaCaT cells for expression of the viral genomes under standardized conditions. We identified 25 miRNAs which were differentially regulated in two or three HPV types where 13 miRNAs were in common for all three types. Among the miRNAs identified, miR-125a-5p, miR-129-3p, miR-363, and miR-145 are related to human cancers. Noteworthy, miR-145 is found upregulated in the miRNA profiles of both high-risk HPV types. For selected differentially expressed miRNAs in HPV 16 predicted miRNA target transcript involved in signal transduction, RNA splicing and tumor invasive growth were validated by qRT-PCR. In addition, our results imply that the early 3' untranslated region (3'UTR) of the three HPV genomes were not a target for miRNA regulation.",
keywords = "3' Untranslated Regions, Cell Line, Gene Expression Regulation, Viral, Genome, Viral, Human papillomavirus 11, Human papillomavirus 16, Humans, MicroRNAs, RNA Splicing, Transfection, Tumor Virus Infections",
author = "Anita Dreher and Maria Rossing and Bogumil Kaczkowski and Andersen, {Ditte K} and Larsen, {Therese Juhlin} and Christophersen, {Mikael Kronborg} and Nielsen, {Finn Cilius} and Bodil Norrild",
note = "Copyright {\circledC} 2011 Elsevier Inc. All rights reserved.",
year = "2011",
doi = "10.1016/j.bbrc.2011.07.011",
language = "English",
volume = "412",
pages = "20--5",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Differential expression of cellular microRNAs in HPV 11, -16, and -45 transfected cells

AU - Dreher, Anita

AU - Rossing, Maria

AU - Kaczkowski, Bogumil

AU - Andersen, Ditte K

AU - Larsen, Therese Juhlin

AU - Christophersen, Mikael Kronborg

AU - Nielsen, Finn Cilius

AU - Norrild, Bodil

N1 - Copyright © 2011 Elsevier Inc. All rights reserved.

PY - 2011

Y1 - 2011

N2 - Human papillomaviruses (HPVs) are highly prevalent giving rise to both benign and malignant lesions why they are classified as high- and low-risk viruses. In this study we selected one low-risk (HPV 11) and two high-risk (HPV 16 and -45) types for genomewide miRNA analysis to investigate possible common and distinct features in the expression profiles. For this purpose we developed a cell culture model system in HaCaT cells for expression of the viral genomes under standardized conditions. We identified 25 miRNAs which were differentially regulated in two or three HPV types where 13 miRNAs were in common for all three types. Among the miRNAs identified, miR-125a-5p, miR-129-3p, miR-363, and miR-145 are related to human cancers. Noteworthy, miR-145 is found upregulated in the miRNA profiles of both high-risk HPV types. For selected differentially expressed miRNAs in HPV 16 predicted miRNA target transcript involved in signal transduction, RNA splicing and tumor invasive growth were validated by qRT-PCR. In addition, our results imply that the early 3' untranslated region (3'UTR) of the three HPV genomes were not a target for miRNA regulation.

AB - Human papillomaviruses (HPVs) are highly prevalent giving rise to both benign and malignant lesions why they are classified as high- and low-risk viruses. In this study we selected one low-risk (HPV 11) and two high-risk (HPV 16 and -45) types for genomewide miRNA analysis to investigate possible common and distinct features in the expression profiles. For this purpose we developed a cell culture model system in HaCaT cells for expression of the viral genomes under standardized conditions. We identified 25 miRNAs which were differentially regulated in two or three HPV types where 13 miRNAs were in common for all three types. Among the miRNAs identified, miR-125a-5p, miR-129-3p, miR-363, and miR-145 are related to human cancers. Noteworthy, miR-145 is found upregulated in the miRNA profiles of both high-risk HPV types. For selected differentially expressed miRNAs in HPV 16 predicted miRNA target transcript involved in signal transduction, RNA splicing and tumor invasive growth were validated by qRT-PCR. In addition, our results imply that the early 3' untranslated region (3'UTR) of the three HPV genomes were not a target for miRNA regulation.

KW - 3' Untranslated Regions

KW - Cell Line

KW - Gene Expression Regulation, Viral

KW - Genome, Viral

KW - Human papillomavirus 11

KW - Human papillomavirus 16

KW - Humans

KW - MicroRNAs

KW - RNA Splicing

KW - Transfection

KW - Tumor Virus Infections

U2 - 10.1016/j.bbrc.2011.07.011

DO - 10.1016/j.bbrc.2011.07.011

M3 - Journal article

C2 - 21782796

VL - 412

SP - 20

EP - 25

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -

ID: 35077640