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Directed differentiation of porcine epiblast-derived neural progenitor cells into neurons and glia

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Neural progenitor cells (NPCs) are promising candidates for cell-based therapy of neurodegenerative diseases; however, safety concerns must be addressed through transplantation studies in large animal models, such as the pig. The aim of this study was to derive NPCs from porcine blastocysts and evaluate their in-vitro differentiation potential. Epiblasts were manually isolated from expanded hatched blastocysts and cultured on MEF feeder cells. Outgrowth colonies were passaged to MS5 cells and rosettes were further passaged to Matrigel-coated dishes containing bFGF and EGF. Three NPC lines were established which showed expression of SOX2, NESTIN and VIMENTIN. One line was characterised in more detail, retaining a normal karyotype and proliferating for more than three months in culture. Following differentiation, TUJI was significantly up-regulated in protocol 2 (RA and SHH; 58% positive cells) as were NF and TH. In contrast, MBP was significantly up-regulated in protocol 3 (FGF8 and SHH; 63% positive cells), whereas, GFAP was significantly up-regulated in protocols 1-4 (33%, 25%, 43% and 22%). The present study provides the first report importance for future transplantation studies in large animal models of neurodegenerative diseases.

OriginalsprogEngelsk
TidsskriftStem Cell Research
Vol/bind7
Udgave nummer2
Sider (fra-til)124-136
Antal sider13
ISSN1873-5061
DOI
StatusUdgivet - 2011

ID: 33772653