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Drug delivery by phospholipase A2 degradable liposomes

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

The effect of poly(ethylene glycol)-phospholipid (PE-PEG) lipopolymers on phospholipase A2 (PLA2) hydrolysis of liposomes composed of stearoyl-oleoylphosphatidylcholine (SOPC) was investigated. The PLA2 lag-time, which is inversely related to the enzymatic activity, was determined by fluorescence, and the zeta-potentials of the liposomes were measured as a function of PE-PEG lipopolymer concentration. A significant decrease in the lag-time, and hence an increase in enzymatic activity, was observed with increasing amounts of the negatively charged PE-PEG lipopolymers incorporated into the SOPC liposomes. The enhancement of the PLA2 enzymatic activity might involve a stronger PLA2 binding affinity towards the negatively charged and polymer covered PEG liposomes.

OriginalsprogEngelsk
TidsskriftInternational Journal of Pharmaceutics
Vol/bind214
Udgave nummer1-2
Sider (fra-til)67-69
Antal sider3
ISSN0378-5173
DOI
StatusUdgivet - 19 feb. 2001

ID: 230987712