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Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells. / Petersen, Elisabeth D.; Billestrup, N; Nielsen, Jens Høiriis.

I: Biomedica Biochimica Acta, Bind 49, Nr. 12, 1990, s. 1171-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Petersen, ED, Billestrup, N & Nielsen, JH 1990, 'Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells', Biomedica Biochimica Acta, bind 49, nr. 12, s. 1171-5.

APA

Petersen, E. D., Billestrup, N., & Nielsen, J. H. (1990). Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells. Biomedica Biochimica Acta, 49(12), 1171-5.

Vancouver

Petersen ED, Billestrup N, Nielsen JH. Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells. Biomedica Biochimica Acta. 1990;49(12):1171-5.

Author

Petersen, Elisabeth D. ; Billestrup, N ; Nielsen, Jens Høiriis. / Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells. I: Biomedica Biochimica Acta. 1990 ; Bind 49, Nr. 12. s. 1171-5.

Bibtex

@article{edd8389951b547ebb6aa840aeed76d33,
title = "Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells",
abstract = "Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5{\%} FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.",
keywords = "Adenylate Cyclase, Animals, Blood, Cycloheximide, DNA Probes, DNA-Binding Proteins, Enzyme Activation, Forskolin, Gene Expression Regulation, Insulin, Insulinoma, Phorbol 12,13-Dibutyrate, Protein Kinase C, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-fos, Proto-Oncogene Proteins c-jun, RNA, Messenger, Rats, Somatomedins, Transcription Factors, Tumor Cells, Cultured",
author = "Petersen, {Elisabeth D.} and N Billestrup and Nielsen, {Jens H{\o}iriis}",
year = "1990",
language = "English",
volume = "49",
pages = "1171--5",
journal = "Biomedica Biochimica Acta",
issn = "0232-766X",
publisher = "Akademie Verlag GMBH",
number = "12",

}

RIS

TY - JOUR

T1 - Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells

AU - Petersen, Elisabeth D.

AU - Billestrup, N

AU - Nielsen, Jens Høiriis

PY - 1990

Y1 - 1990

N2 - Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.

AB - Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.

KW - Adenylate Cyclase

KW - Animals

KW - Blood

KW - Cycloheximide

KW - DNA Probes

KW - DNA-Binding Proteins

KW - Enzyme Activation

KW - Forskolin

KW - Gene Expression Regulation

KW - Insulin

KW - Insulinoma

KW - Phorbol 12,13-Dibutyrate

KW - Protein Kinase C

KW - Protein-Tyrosine Kinases

KW - Proto-Oncogene Proteins

KW - Proto-Oncogene Proteins c-fos

KW - Proto-Oncogene Proteins c-jun

KW - RNA, Messenger

KW - Rats

KW - Somatomedins

KW - Transcription Factors

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 2129102

VL - 49

SP - 1171

EP - 1175

JO - Biomedica Biochimica Acta

JF - Biomedica Biochimica Acta

SN - 0232-766X

IS - 12

ER -

ID: 47974133