Forskning ved Københavns Universitet - Københavns Universitet


Elevated miR-615-3p Expression Predicts Adverse Clinical Outcome and Promotes Proliferation and Migration of Prostate Cancer Cells

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt


  • Emma B Laursen
  • Jacob Fredsøe
  • Linnéa Schmidt
  • Siri H Strand
  • Helle Kristensen
  • Anne K I Rasmussen
  • Tina F Daugaard
  • Peter Mouritzen
  • Søren Høyer
  • Gitte Kristensen
  • Hein V Stroomberg
  • Brasso, Klaus
  • Røder, Martin Andreas
  • Michael Borre
  • Karina D Sørensen

miR-615-3p has previously been described as up-regulated in prostate cancer (PC) tissue samples compared with nonmalignant controls; however, its prognostic potential and functional role in PC remain largely unknown. In this study, we investigated the clinical and biological relevance of miR-615-3p in PC. The expression of miR-615-3p was measured in PC tissue specimens from 239 men who underwent radical prostatectomy (RP), and it was investigated if miR-615-3p could predict postoperative biochemical recurrence (BCR). These findings were subsequently validated in three independent RP cohorts (n = 222, n = 273, and n = 387) and functional overexpression studies conducted in PC cells (PC3M). High miR-615-3p expression was significantly associated with BCR in four independent PC patient cohorts (P < 0.05, log-rank test). In addition, high miR-615-3p expression was a significant predictor of PC-specific survival in univariate (hazard ratio, 3.75; P < 0.001) and multivariate (hazard ratio, 2.66; P = 0.008) analysis after adjustment for the Cancer of the Prostate Risk Assessment Post-Surgical (CAPRA-S) nomogram in a merged RP cohort (n = 734). Moreover, overexpression of miR-615-3p in PC cells (PC3M) significantly increased cell viability, proliferation, apoptosis, and migration. Together, our results suggest that miR-615-3p is a significant predictor of postoperative BCR and PC-specific survival and has oncogenic functions in PC cells.

TidsskriftThe American Journal of Pathology
Udgave nummer12
Sider (fra-til)2377-2388
Antal sider12
StatusUdgivet - dec. 2019

Bibliografisk note

Copyright © 2019 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.

ID: 237189193