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Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats

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Standard

Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats. / Bukhave, K.; Hansen, Harald S.

I: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Bind 489, Nr. 3, 21.12.1977, s. 403-414.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bukhave, K & Hansen, HS 1977, 'Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats', Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, bind 489, nr. 3, s. 403-414.

APA

Bukhave, K., & Hansen, H. S. (1977). Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 489(3), 403-414.

Vancouver

Bukhave K, Hansen HS. Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1977 dec 21;489(3):403-414.

Author

Bukhave, K. ; Hansen, Harald S. / Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats. I: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1977 ; Bind 489, Nr. 3. s. 403-414.

Bibtex

@article{e7dc055dce1a41d0a6274df4640186e0,
title = "Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats",
abstract = "The elimination of [H]prostaglandin E, in anaesthetized rats was studied by continuous intravenous or intraarterial infusions, producing steady-state concentrations at the level of endogenous prostaglandin E in mixed venous blood. Blood samples (0.5 ml) were collected from the carotid artery or the right atrium, respectively. The levels of [H]prostaglandin E were measured at different infusion time intervals and the H-labeled hydrophobic metabolites characterized. Cardiac output was estimated by a modification of the dye injection method, using I-labelled albumin as the marker. From the cardiac output and the rate of infusion, the fractional clearance of the lung and the systemic beds in the steady-state situation were estimated to 88.3 ± 3.2{\%} and 54.1 ± 15.2{\%}(mean ± S.D.), respectively. The hydrophobic metabolites were characterized chromatographically on Sephadex LH-20 columns, using synthetically prepared [C]prostaglandin metabolites as internal standards and markers. The identities of some metabolites were further established by derivative formation to a constant [H]/[C] ratio. The major metabolite was 15-keto-13,14-dihydro-[H]prostaglandin E, while 15-keto-[H]prostaglandin E and 13,14-dihydro-[H]prostaglandin E could not be demonstrated.",
author = "K. Bukhave and Hansen, {Harald S.}",
year = "1977",
month = "12",
day = "21",
language = "English",
volume = "489",
pages = "403--414",
journal = "Biochimica et Biophysica Acta - Lipids and Lipid Metabolism",
issn = "0005-2760",
publisher = "Elsevier",
number = "3",

}

RIS

TY - JOUR

T1 - Elimination of low steady-state concentrations of [5,6-H]prostaglandin E in the pulmonary and the systemic circulations of anaesthetized rats

AU - Bukhave, K.

AU - Hansen, Harald S.

PY - 1977/12/21

Y1 - 1977/12/21

N2 - The elimination of [H]prostaglandin E, in anaesthetized rats was studied by continuous intravenous or intraarterial infusions, producing steady-state concentrations at the level of endogenous prostaglandin E in mixed venous blood. Blood samples (0.5 ml) were collected from the carotid artery or the right atrium, respectively. The levels of [H]prostaglandin E were measured at different infusion time intervals and the H-labeled hydrophobic metabolites characterized. Cardiac output was estimated by a modification of the dye injection method, using I-labelled albumin as the marker. From the cardiac output and the rate of infusion, the fractional clearance of the lung and the systemic beds in the steady-state situation were estimated to 88.3 ± 3.2% and 54.1 ± 15.2%(mean ± S.D.), respectively. The hydrophobic metabolites were characterized chromatographically on Sephadex LH-20 columns, using synthetically prepared [C]prostaglandin metabolites as internal standards and markers. The identities of some metabolites were further established by derivative formation to a constant [H]/[C] ratio. The major metabolite was 15-keto-13,14-dihydro-[H]prostaglandin E, while 15-keto-[H]prostaglandin E and 13,14-dihydro-[H]prostaglandin E could not be demonstrated.

AB - The elimination of [H]prostaglandin E, in anaesthetized rats was studied by continuous intravenous or intraarterial infusions, producing steady-state concentrations at the level of endogenous prostaglandin E in mixed venous blood. Blood samples (0.5 ml) were collected from the carotid artery or the right atrium, respectively. The levels of [H]prostaglandin E were measured at different infusion time intervals and the H-labeled hydrophobic metabolites characterized. Cardiac output was estimated by a modification of the dye injection method, using I-labelled albumin as the marker. From the cardiac output and the rate of infusion, the fractional clearance of the lung and the systemic beds in the steady-state situation were estimated to 88.3 ± 3.2% and 54.1 ± 15.2%(mean ± S.D.), respectively. The hydrophobic metabolites were characterized chromatographically on Sephadex LH-20 columns, using synthetically prepared [C]prostaglandin metabolites as internal standards and markers. The identities of some metabolites were further established by derivative formation to a constant [H]/[C] ratio. The major metabolite was 15-keto-13,14-dihydro-[H]prostaglandin E, while 15-keto-[H]prostaglandin E and 13,14-dihydro-[H]prostaglandin E could not be demonstrated.

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M3 - Journal article

AN - SCOPUS:0017749517

VL - 489

SP - 403

EP - 414

JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

SN - 0005-2760

IS - 3

ER -

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