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Environmental factors affecting the expression of type IV pilus genes as well as piliation of Thermus thermophilus

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  • Ralf Salzer
  • Timo Kern
  • Friederike Joos
  • Beate Averhoff

The thermophilic bacterium Thermus thermophilus HB27 is known for its highly efficient natural transformation system, which has become a model system to study the structure and function of DNA transporter in thermophilic bacteria. The DNA transporter is functionally linked to type IV pili (T4P), which are essential for twitching motility and adhesion to solid surfaces. However, the pilus structures themselves are dispensable for natural transformation. Here, we report that the cellular mRNA levels of the major structural subunit of the T4P, PilA4, are regulated by environmental factors. Growth of T. thermophilus in minimal medium or low temperature (55 °C) leads to a significant increase in pilA4 transcripts. In contrast, the transcript levels of the minor pilin pilA1 as well as other T4P genes are nearly unaffected. The elevated pilA4 mRNA levels are accompanied by an increase in piliation of the cells but not by elevated natural transformation frequencies. Hyperpiliation leads to increased adhesion to plastic surfaces. The increased cell-surface interactions are suggested to represent an adaptive response to temperature stress and may be advantageous for survival of T. thermophilus.

OriginalsprogEngelsk
TidsskriftFEMS Microbiology Letters
Vol/bind357
Udgave nummer1
Sider (fra-til)56-62
Antal sider7
ISSN0378-1097
DOI
StatusUdgivet - aug. 2014

ID: 196441768