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Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy. / von Walden, Ferdinand; Fernandez-Gonzalo, Rodrigo; Pingel, Jessica; McCarthy, John; Stål, Per; Pontén, Eva.

I: Frontiers in Pediatrics, Bind 8, 236, 2020.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

von Walden, F, Fernandez-Gonzalo, R, Pingel, J, McCarthy, J, Stål, P & Pontén, E 2020, 'Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy', Frontiers in Pediatrics, bind 8, 236. https://doi.org/10.3389/fped.2020.00236

APA

von Walden, F., Fernandez-Gonzalo, R., Pingel, J., McCarthy, J., Stål, P., & Pontén, E. (2020). Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy. Frontiers in Pediatrics, 8, [236]. https://doi.org/10.3389/fped.2020.00236

Vancouver

von Walden F, Fernandez-Gonzalo R, Pingel J, McCarthy J, Stål P, Pontén E. Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy. Frontiers in Pediatrics. 2020;8. 236. https://doi.org/10.3389/fped.2020.00236

Author

von Walden, Ferdinand ; Fernandez-Gonzalo, Rodrigo ; Pingel, Jessica ; McCarthy, John ; Stål, Per ; Pontén, Eva. / Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy. I: Frontiers in Pediatrics. 2020 ; Bind 8.

Bibtex

@article{76e5128943d04086b1d45a5b3186044a,
title = "Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy",
abstract = "Introduction: Cerebral palsy (CP) is the most common motor impairment in children. Skeletal muscles in individuals with CP are typically weak, thin, and stiff. Whether epigenetic changes at the ribosomal DNA (rDNA) promoter are involved in this dysregulation remains unknown. Methods: Skeletal muscle samples were collected from 19 children with CP and 10 typically developed (TD) control children. Methylation of the rDNA promoter was analyzed using the Agena Epityper Mass array and gene expression by qRT-PCR. Results: Biceps brachii muscle ribosome biogenesis was suppressed in CP as compared to TD. Average methylation of the rDNA promoter was not different between CP and TD but negatively correlated to elbow flexor contracture in the CP group. Discussions: We observed a negative correlation between rDNA promoter methylation and degree of muscle contracture in the CP group. Children with CP with more severe motor impairment had less methylation of the rDNA promoter compared to less affected children. This finding suggests the importance of neural input and voluntary muscle movements for promoter methylation to occur in the biceps muscle.",
keywords = "cerebral palsy, DNA methylation, epigenetics, ribosome biogenesis, skeletal muscle",
author = "{von Walden}, Ferdinand and Rodrigo Fernandez-Gonzalo and Jessica Pingel and John McCarthy and Per St{\aa}l and Eva Pont{\'e}n",
year = "2020",
doi = "10.3389/fped.2020.00236",
language = "English",
volume = "8",
journal = "Frontiers in Pediatrics",
issn = "2296-2360",
publisher = "Frontiers Media",

}

RIS

TY - JOUR

T1 - Epigenetic Marks at the Ribosomal DNA Promoter in Skeletal Muscle Are Negatively Associated With Degree of Impairment in Cerebral Palsy

AU - von Walden, Ferdinand

AU - Fernandez-Gonzalo, Rodrigo

AU - Pingel, Jessica

AU - McCarthy, John

AU - Stål, Per

AU - Pontén, Eva

PY - 2020

Y1 - 2020

N2 - Introduction: Cerebral palsy (CP) is the most common motor impairment in children. Skeletal muscles in individuals with CP are typically weak, thin, and stiff. Whether epigenetic changes at the ribosomal DNA (rDNA) promoter are involved in this dysregulation remains unknown. Methods: Skeletal muscle samples were collected from 19 children with CP and 10 typically developed (TD) control children. Methylation of the rDNA promoter was analyzed using the Agena Epityper Mass array and gene expression by qRT-PCR. Results: Biceps brachii muscle ribosome biogenesis was suppressed in CP as compared to TD. Average methylation of the rDNA promoter was not different between CP and TD but negatively correlated to elbow flexor contracture in the CP group. Discussions: We observed a negative correlation between rDNA promoter methylation and degree of muscle contracture in the CP group. Children with CP with more severe motor impairment had less methylation of the rDNA promoter compared to less affected children. This finding suggests the importance of neural input and voluntary muscle movements for promoter methylation to occur in the biceps muscle.

AB - Introduction: Cerebral palsy (CP) is the most common motor impairment in children. Skeletal muscles in individuals with CP are typically weak, thin, and stiff. Whether epigenetic changes at the ribosomal DNA (rDNA) promoter are involved in this dysregulation remains unknown. Methods: Skeletal muscle samples were collected from 19 children with CP and 10 typically developed (TD) control children. Methylation of the rDNA promoter was analyzed using the Agena Epityper Mass array and gene expression by qRT-PCR. Results: Biceps brachii muscle ribosome biogenesis was suppressed in CP as compared to TD. Average methylation of the rDNA promoter was not different between CP and TD but negatively correlated to elbow flexor contracture in the CP group. Discussions: We observed a negative correlation between rDNA promoter methylation and degree of muscle contracture in the CP group. Children with CP with more severe motor impairment had less methylation of the rDNA promoter compared to less affected children. This finding suggests the importance of neural input and voluntary muscle movements for promoter methylation to occur in the biceps muscle.

KW - cerebral palsy

KW - DNA methylation

KW - epigenetics

KW - ribosome biogenesis

KW - skeletal muscle

U2 - 10.3389/fped.2020.00236

DO - 10.3389/fped.2020.00236

M3 - Journal article

C2 - 32582584

AN - SCOPUS:85086776823

VL - 8

JO - Frontiers in Pediatrics

JF - Frontiers in Pediatrics

SN - 2296-2360

M1 - 236

ER -

ID: 244320475