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Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice

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Standard

Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice. / Ranne, Ebbe; Hansen, Gunilla H.; Behrendt, Niels; Solberg, Hélène; Larsen, Jørgen K.; Danø, Keld.

I: Fibrinolysis and Proteolysis, Bind 11, Nr. SUPPL. 3, 01.12.1997.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Ranne, E, Hansen, GH, Behrendt, N, Solberg, H, Larsen, JK & Danø, K 1997, 'Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice', Fibrinolysis and Proteolysis, bind 11, nr. SUPPL. 3.

APA

Ranne, E., Hansen, G. H., Behrendt, N., Solberg, H., Larsen, J. K., & Danø, K. (1997). Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice. Fibrinolysis and Proteolysis, 11(SUPPL. 3).

Vancouver

Ranne E, Hansen GH, Behrendt N, Solberg H, Larsen JK, Danø K. Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice. Fibrinolysis and Proteolysis. 1997 dec 1;11(SUPPL. 3).

Author

Ranne, Ebbe ; Hansen, Gunilla H. ; Behrendt, Niels ; Solberg, Hélène ; Larsen, Jørgen K. ; Danø, Keld. / Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice. I: Fibrinolysis and Proteolysis. 1997 ; Bind 11, Nr. SUPPL. 3.

Bibtex

@article{68169835c8694504aeaec41870a939b8,
title = "Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice",
abstract = "The urokinase plasminogen activator receptor (uPAR) plays a central role during cancer invasion and metastasis by localizing and focalizing the uPA mediated proteolytic activity to the cell surface.We have previously raised monoclonal antibodies to the human uPAR (Renne, E. et al (1991) FEBS Lett. 288, 233-236) and used them for different analysis of human uPAR. None of the antibodies showed any cross-reactivity to murine uPAR. In order to study the murine uPAR further we immunized uPAR knock-out mice ( Bugge, T.H. et al (1995) J. Biol. Chem. 270, 16886-16894) with human soluble uPAR, based on the assumption that it might be possible to raise antibodies to epitopes highly conserved between human and mouse. From one fusion we obtained 22 stable hybridomas. Two of the hybridomas produced antibodies that cross-react with the murin uPAR as analysed by immunoblotting using Tx-114 detergent phase extract from a murine cell line. Further analysis of the antibodies showed that the react with an epitop on domain 2+3 of uPAR and only under non-reducing conditions of the antigen. The antibodies also showed useful for immunoaffinity purification of soluble murin uPAR as well as staining of uPAR on cells as demonstrated by flow cytometry analysis. In conclusion , we have demonstrated that it is possible to raise antibodies to highly conserved epitopes between human and mouse uPAR by immunizing uPAR knock-out mice with human uPAR. The antibodies generated might be a useful tool for demonstrating the occurence and amount of rnurine uPAR in different biological samples but further studies are needed.",
author = "Ebbe Ranne and Hansen, {Gunilla H.} and Niels Behrendt and H{\'e}l{\`e}ne Solberg and Larsen, {J{\o}rgen K.} and Keld Dan{\o}",
year = "1997",
month = dec,
day = "1",
language = "English",
volume = "11",
journal = "Fibrinolysis & proteolysis",
issn = "1369-0191",
publisher = "Churchill Livingstone",
number = "SUPPL. 3",

}

RIS

TY - JOUR

T1 - Establishment of murine monoclonal antibodies against murine urokinase receptor raised in urokinase receptor knock-out mice

AU - Ranne, Ebbe

AU - Hansen, Gunilla H.

AU - Behrendt, Niels

AU - Solberg, Hélène

AU - Larsen, Jørgen K.

AU - Danø, Keld

PY - 1997/12/1

Y1 - 1997/12/1

N2 - The urokinase plasminogen activator receptor (uPAR) plays a central role during cancer invasion and metastasis by localizing and focalizing the uPA mediated proteolytic activity to the cell surface.We have previously raised monoclonal antibodies to the human uPAR (Renne, E. et al (1991) FEBS Lett. 288, 233-236) and used them for different analysis of human uPAR. None of the antibodies showed any cross-reactivity to murine uPAR. In order to study the murine uPAR further we immunized uPAR knock-out mice ( Bugge, T.H. et al (1995) J. Biol. Chem. 270, 16886-16894) with human soluble uPAR, based on the assumption that it might be possible to raise antibodies to epitopes highly conserved between human and mouse. From one fusion we obtained 22 stable hybridomas. Two of the hybridomas produced antibodies that cross-react with the murin uPAR as analysed by immunoblotting using Tx-114 detergent phase extract from a murine cell line. Further analysis of the antibodies showed that the react with an epitop on domain 2+3 of uPAR and only under non-reducing conditions of the antigen. The antibodies also showed useful for immunoaffinity purification of soluble murin uPAR as well as staining of uPAR on cells as demonstrated by flow cytometry analysis. In conclusion , we have demonstrated that it is possible to raise antibodies to highly conserved epitopes between human and mouse uPAR by immunizing uPAR knock-out mice with human uPAR. The antibodies generated might be a useful tool for demonstrating the occurence and amount of rnurine uPAR in different biological samples but further studies are needed.

AB - The urokinase plasminogen activator receptor (uPAR) plays a central role during cancer invasion and metastasis by localizing and focalizing the uPA mediated proteolytic activity to the cell surface.We have previously raised monoclonal antibodies to the human uPAR (Renne, E. et al (1991) FEBS Lett. 288, 233-236) and used them for different analysis of human uPAR. None of the antibodies showed any cross-reactivity to murine uPAR. In order to study the murine uPAR further we immunized uPAR knock-out mice ( Bugge, T.H. et al (1995) J. Biol. Chem. 270, 16886-16894) with human soluble uPAR, based on the assumption that it might be possible to raise antibodies to epitopes highly conserved between human and mouse. From one fusion we obtained 22 stable hybridomas. Two of the hybridomas produced antibodies that cross-react with the murin uPAR as analysed by immunoblotting using Tx-114 detergent phase extract from a murine cell line. Further analysis of the antibodies showed that the react with an epitop on domain 2+3 of uPAR and only under non-reducing conditions of the antigen. The antibodies also showed useful for immunoaffinity purification of soluble murin uPAR as well as staining of uPAR on cells as demonstrated by flow cytometry analysis. In conclusion , we have demonstrated that it is possible to raise antibodies to highly conserved epitopes between human and mouse uPAR by immunizing uPAR knock-out mice with human uPAR. The antibodies generated might be a useful tool for demonstrating the occurence and amount of rnurine uPAR in different biological samples but further studies are needed.

UR - http://www.scopus.com/inward/record.url?scp=33846705360&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:33846705360

VL - 11

JO - Fibrinolysis & proteolysis

JF - Fibrinolysis & proteolysis

SN - 1369-0191

IS - SUPPL. 3

ER -

ID: 241481146