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Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production

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Standard

Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production. / Lindedam, Jane; Bruun, Sander; Jørgensen, Henning; Decker, Stephen R.; Turner, Geoffrey B.; DeMartini, Jaclyn D.; Wyman, Charles E.; Felby, Claus.

I: Biomass & Bioenergy, Bind 66, 2014, s. 261-267.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Lindedam, J, Bruun, S, Jørgensen, H, Decker, SR, Turner, GB, DeMartini, JD, Wyman, CE & Felby, C 2014, 'Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production', Biomass & Bioenergy, bind 66, s. 261-267. https://doi.org/10.1016/j.biombioe.2014.03.006

APA

Lindedam, J., Bruun, S., Jørgensen, H., Decker, S. R., Turner, G. B., DeMartini, J. D., ... Felby, C. (2014). Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production. Biomass & Bioenergy, 66, 261-267. https://doi.org/10.1016/j.biombioe.2014.03.006

Vancouver

Lindedam J, Bruun S, Jørgensen H, Decker SR, Turner GB, DeMartini JD o.a. Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production. Biomass & Bioenergy. 2014;66:261-267. https://doi.org/10.1016/j.biombioe.2014.03.006

Author

Lindedam, Jane ; Bruun, Sander ; Jørgensen, Henning ; Decker, Stephen R. ; Turner, Geoffrey B. ; DeMartini, Jaclyn D. ; Wyman, Charles E. ; Felby, Claus. / Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production. I: Biomass & Bioenergy. 2014 ; Bind 66. s. 261-267.

Bibtex

@article{f411815c081a4e5a9b5cb064c15790e5,
title = "Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production",
abstract = "We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57{\%}, 64{\%}, and 71{\%} from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R2=0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2=0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.",
keywords = "Genetic selection, High throughput screening, Lignocellulosic ethanol, Microscale pretreatment, Wheat straw",
author = "Jane Lindedam and Sander Bruun and Henning J{\o}rgensen and Decker, {Stephen R.} and Turner, {Geoffrey B.} and DeMartini, {Jaclyn D.} and Wyman, {Charles E.} and Claus Felby",
year = "2014",
doi = "10.1016/j.biombioe.2014.03.006",
language = "English",
volume = "66",
pages = "261--267",
journal = "Biomass & Bioenergy",
issn = "0961-9534",
publisher = "Pergamon Press",

}

RIS

TY - JOUR

T1 - Evaluation of high throughput screening methods in picking up differences between cultivars of lignocellulosic biomass for ethanol production

AU - Lindedam, Jane

AU - Bruun, Sander

AU - Jørgensen, Henning

AU - Decker, Stephen R.

AU - Turner, Geoffrey B.

AU - DeMartini, Jaclyn D.

AU - Wyman, Charles E.

AU - Felby, Claus

PY - 2014

Y1 - 2014

N2 - We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R2=0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2=0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.

AB - We present a unique evaluation of three advanced high throughput pretreatment and enzymatic hydrolysis systems (HTPH-systems) for screening of lignocellulosic biomass for enzymatic saccharification. Straw from 20 cultivars of winter wheat from two sites in Denmark was hydrothermally pretreated and enzymatically processed in each of the separately engineered HTPH-systems at 1) University of California, Riverside, 2) National Renewable Energy Laboratory (NREL), Colorado, and 3) University of Copenhagen (CPH). All three systems were able to detect significant differences between the cultivars in the release of fermentable sugars, with average cellulose conversions of 57%, 64%, and 71% from Riverside, NREL and CPH, respectively. The best correlation of glucose yields was found between the Riverside and NREL systems (R2=0.2139), and the best correlation for xylose yields was found between Riverside and CPH (R2=0.4269). All three systems identified Flair as the highest yielding cultivar and Dinosor, Glasgow, and Robigus as low yielding cultivars. Despite different conditions in the three HTPH-systems, the approach of microscale screening for phenotypically less recalcitrant feedstock seems sufficiently robust to be used as a generic analytical platform.

KW - Genetic selection

KW - High throughput screening

KW - Lignocellulosic ethanol

KW - Microscale pretreatment

KW - Wheat straw

U2 - 10.1016/j.biombioe.2014.03.006

DO - 10.1016/j.biombioe.2014.03.006

M3 - Journal article

AN - SCOPUS:84901640759

VL - 66

SP - 261

EP - 267

JO - Biomass & Bioenergy

JF - Biomass & Bioenergy

SN - 0961-9534

ER -

ID: 130102152