Forskning ved Københavns Universitet - Københavns Universitet

Forside

Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec)

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec). / Rolo, Joana; Worning, Peder; Nielsen, Jesper Boye; Bowden, Rory; Bouchami, Ons; Damborg, Peter; Guardabassi, Luca; Perreten, Vincent; Tomasz, Alexander; Westh, Henrik; de Lencastre, Hermínia; Miragaia, Maria.

I: Antimicrobial Agents and Chemotherapy, Bind 61, Nr. 6, e02302-16, 2017.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Rolo, J, Worning, P, Nielsen, JB, Bowden, R, Bouchami, O, Damborg, P, Guardabassi, L, Perreten, V, Tomasz, A, Westh, H, de Lencastre, H & Miragaia, M 2017, 'Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec)', Antimicrobial Agents and Chemotherapy, bind 61, nr. 6, e02302-16. https://doi.org/10.1128/AAC.02302-16

APA

Rolo, J., Worning, P., Nielsen, J. B., Bowden, R., Bouchami, O., Damborg, P., ... Miragaia, M. (2017). Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec). Antimicrobial Agents and Chemotherapy, 61(6), [e02302-16]. https://doi.org/10.1128/AAC.02302-16

Vancouver

Rolo J, Worning P, Nielsen JB, Bowden R, Bouchami O, Damborg P o.a. Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec). Antimicrobial Agents and Chemotherapy. 2017;61(6). e02302-16. https://doi.org/10.1128/AAC.02302-16

Author

Rolo, Joana ; Worning, Peder ; Nielsen, Jesper Boye ; Bowden, Rory ; Bouchami, Ons ; Damborg, Peter ; Guardabassi, Luca ; Perreten, Vincent ; Tomasz, Alexander ; Westh, Henrik ; de Lencastre, Hermínia ; Miragaia, Maria. / Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec). I: Antimicrobial Agents and Chemotherapy. 2017 ; Bind 61, Nr. 6.

Bibtex

@article{dbc683d678864e9e96f2812b33a3bd9d,
title = "Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec)",
abstract = "Several lines of evidence indicate that the most primitive staphylococcal species, those of the Staphylococcus sciuri group, were involved in the first stages of evolution of the staphylococcal cassette chromosome mec (SCCmec), the genetic element carrying the β-lactam resistance gene mecA. However, many steps are still missing from this evolutionary history. In particular, it is not known how mecA was incorporated into the mobile element SCC prior to dissemination among Staphylococcus aureus and other pathogenic staphylococcal species. To gain insights into the possible contribution of several species of the Staphylococcus sciuri group to the assembly of SCCmec, we sequenced the genomes of 106 isolates, comprising S. sciuri (n = 76), Staphylococcus vitulinus (n = 18), and Staphylococcus fleurettii (n = 12) from animal and human sources, and characterized the native location of mecA and the SCC insertion site by using a variety of comparative genomic approaches. Moreover, we performed a single nucleotide polymorphism (SNP) analysis of the genomes in order to understand SCCmec evolution in relation to phylogeny. We found that each of three species of the S. sciuri group contributed to the evolution of SCCmec: S. vitulinus and S. fleurettii contributed to the assembly of the mec complex, and S. sciuri most likely provided the mobile element in which mecA was later incorporated. We hypothesize that an ancestral SCCmec III cassette (an element carried by one of the most epidemic methicillin-resistant S. aureus clones) originated in S. sciuri possibly by a recombination event in a human host or a human-created environment and later was transferred to S. aureus.",
keywords = "SCCmec, beta-lactam resistance, evolution, mecA, staphylococci",
author = "Joana Rolo and Peder Worning and Nielsen, {Jesper Boye} and Rory Bowden and Ons Bouchami and Peter Damborg and Luca Guardabassi and Vincent Perreten and Alexander Tomasz and Henrik Westh and {de Lencastre}, Herm{\'i}nia and Maria Miragaia",
year = "2017",
doi = "10.1128/AAC.02302-16",
language = "English",
volume = "61",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "6",

}

RIS

TY - JOUR

T1 - Evolutionary Origin of the Staphylococcal Cassette Chromosome mec (SCCmec)

AU - Rolo, Joana

AU - Worning, Peder

AU - Nielsen, Jesper Boye

AU - Bowden, Rory

AU - Bouchami, Ons

AU - Damborg, Peter

AU - Guardabassi, Luca

AU - Perreten, Vincent

AU - Tomasz, Alexander

AU - Westh, Henrik

AU - de Lencastre, Hermínia

AU - Miragaia, Maria

PY - 2017

Y1 - 2017

N2 - Several lines of evidence indicate that the most primitive staphylococcal species, those of the Staphylococcus sciuri group, were involved in the first stages of evolution of the staphylococcal cassette chromosome mec (SCCmec), the genetic element carrying the β-lactam resistance gene mecA. However, many steps are still missing from this evolutionary history. In particular, it is not known how mecA was incorporated into the mobile element SCC prior to dissemination among Staphylococcus aureus and other pathogenic staphylococcal species. To gain insights into the possible contribution of several species of the Staphylococcus sciuri group to the assembly of SCCmec, we sequenced the genomes of 106 isolates, comprising S. sciuri (n = 76), Staphylococcus vitulinus (n = 18), and Staphylococcus fleurettii (n = 12) from animal and human sources, and characterized the native location of mecA and the SCC insertion site by using a variety of comparative genomic approaches. Moreover, we performed a single nucleotide polymorphism (SNP) analysis of the genomes in order to understand SCCmec evolution in relation to phylogeny. We found that each of three species of the S. sciuri group contributed to the evolution of SCCmec: S. vitulinus and S. fleurettii contributed to the assembly of the mec complex, and S. sciuri most likely provided the mobile element in which mecA was later incorporated. We hypothesize that an ancestral SCCmec III cassette (an element carried by one of the most epidemic methicillin-resistant S. aureus clones) originated in S. sciuri possibly by a recombination event in a human host or a human-created environment and later was transferred to S. aureus.

AB - Several lines of evidence indicate that the most primitive staphylococcal species, those of the Staphylococcus sciuri group, were involved in the first stages of evolution of the staphylococcal cassette chromosome mec (SCCmec), the genetic element carrying the β-lactam resistance gene mecA. However, many steps are still missing from this evolutionary history. In particular, it is not known how mecA was incorporated into the mobile element SCC prior to dissemination among Staphylococcus aureus and other pathogenic staphylococcal species. To gain insights into the possible contribution of several species of the Staphylococcus sciuri group to the assembly of SCCmec, we sequenced the genomes of 106 isolates, comprising S. sciuri (n = 76), Staphylococcus vitulinus (n = 18), and Staphylococcus fleurettii (n = 12) from animal and human sources, and characterized the native location of mecA and the SCC insertion site by using a variety of comparative genomic approaches. Moreover, we performed a single nucleotide polymorphism (SNP) analysis of the genomes in order to understand SCCmec evolution in relation to phylogeny. We found that each of three species of the S. sciuri group contributed to the evolution of SCCmec: S. vitulinus and S. fleurettii contributed to the assembly of the mec complex, and S. sciuri most likely provided the mobile element in which mecA was later incorporated. We hypothesize that an ancestral SCCmec III cassette (an element carried by one of the most epidemic methicillin-resistant S. aureus clones) originated in S. sciuri possibly by a recombination event in a human host or a human-created environment and later was transferred to S. aureus.

KW - SCCmec

KW - beta-lactam resistance

KW - evolution

KW - mecA

KW - staphylococci

U2 - 10.1128/AAC.02302-16

DO - 10.1128/AAC.02302-16

M3 - Journal article

C2 - 28373201

VL - 61

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 6

M1 - e02302-16

ER -

ID: 180504951