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Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma. / Kopp, Katharina L; Ralfkiaer, Ulrik; Nielsen, Boye S; Gniadecki, Robert; Andersen, Anders Woetmann; Ødum, Niels; Ralfkiaer, Elisabeth.

I: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Bind 121, Nr. 11, 11.2013, s. 1020-4.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kopp, KL, Ralfkiaer, U, Nielsen, BS, Gniadecki, R, Andersen, AW, Ødum, N & Ralfkiaer, E 2013, 'Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, bind 121, nr. 11, s. 1020-4. https://doi.org/10.1111/apm.12162

APA

Kopp, K. L., Ralfkiaer, U., Nielsen, B. S., Gniadecki, R., Andersen, A. W., Ødum, N., & Ralfkiaer, E. (2013). Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 121(11), 1020-4. https://doi.org/10.1111/apm.12162

Vancouver

Kopp KL, Ralfkiaer U, Nielsen BS, Gniadecki R, Andersen AW, Ødum N o.a. Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2013 nov;121(11):1020-4. https://doi.org/10.1111/apm.12162

Author

Kopp, Katharina L ; Ralfkiaer, Ulrik ; Nielsen, Boye S ; Gniadecki, Robert ; Andersen, Anders Woetmann ; Ødum, Niels ; Ralfkiaer, Elisabeth. / Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma. I: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2013 ; Bind 121, Nr. 11. s. 1020-4.

Bibtex

@article{b6fbb03fb304495e8484da21af76ed61,
title = "Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma",
abstract = "Recently, miR-155 has been implicated in cutaneous T-cell lymphoma (CTCL). Thus, elevated levels of miR-155 were observed in skin lesions from CTCL patients as judged from qPCR and micro-array analysis and aberrant, high miR-155 expression was associated with severe disease. Moreover, miR-155 promoted proliferation of malignant T cells in vitro. Little is, however, known about which cell types express miR-155 in vivo in CTCL skin lesions. Here, we study miR-155 expression using in situ hybridization (ISH) with a miR-155 probe, a negative control (scrambled), and a miR-126 probe as a positive control in nine patients with mycosis fungoides, the most frequent subtype of CTCL. We provide evidence that both malignant and non-malignant T cells stain weakly to moderately positive with the miR-155 probe, but generally negative with the miR-126 and negative control probes. Reversely, endothelial cells stain positive for miR-126 and negative for miR-155 and the control probe. Solitary T cells with a malignant morphology display brighter staining with the miR-155 probe. Taken together, our findings suggest that both malignant and non-malignant T cells express miR-155 in situ in CTCL. Moreover, they indicate heterogeneity in miR-155 expression among malignant T cells.",
keywords = "Adult, Aged, Aged, 80 and over, Female, Humans, In Situ Hybridization, Lymphoma, T-Cell, Cutaneous, Male, MicroRNAs, Middle Aged, Skin Neoplasms",
author = "Kopp, {Katharina L} and Ulrik Ralfkiaer and Nielsen, {Boye S} and Robert Gniadecki and Andersen, {Anders Woetmann} and Niels {\O}dum and Elisabeth Ralfkiaer",
note = "{\textcopyright} 2013 APMIS. Published by John Wiley & Sons Ltd.",
year = "2013",
month = nov,
doi = "10.1111/apm.12162",
language = "English",
volume = "121",
pages = "1020--4",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "11",

}

RIS

TY - JOUR

T1 - Expression of miR-155 and miR-126 in situ in cutaneous T-cell lymphoma

AU - Kopp, Katharina L

AU - Ralfkiaer, Ulrik

AU - Nielsen, Boye S

AU - Gniadecki, Robert

AU - Andersen, Anders Woetmann

AU - Ødum, Niels

AU - Ralfkiaer, Elisabeth

N1 - © 2013 APMIS. Published by John Wiley & Sons Ltd.

PY - 2013/11

Y1 - 2013/11

N2 - Recently, miR-155 has been implicated in cutaneous T-cell lymphoma (CTCL). Thus, elevated levels of miR-155 were observed in skin lesions from CTCL patients as judged from qPCR and micro-array analysis and aberrant, high miR-155 expression was associated with severe disease. Moreover, miR-155 promoted proliferation of malignant T cells in vitro. Little is, however, known about which cell types express miR-155 in vivo in CTCL skin lesions. Here, we study miR-155 expression using in situ hybridization (ISH) with a miR-155 probe, a negative control (scrambled), and a miR-126 probe as a positive control in nine patients with mycosis fungoides, the most frequent subtype of CTCL. We provide evidence that both malignant and non-malignant T cells stain weakly to moderately positive with the miR-155 probe, but generally negative with the miR-126 and negative control probes. Reversely, endothelial cells stain positive for miR-126 and negative for miR-155 and the control probe. Solitary T cells with a malignant morphology display brighter staining with the miR-155 probe. Taken together, our findings suggest that both malignant and non-malignant T cells express miR-155 in situ in CTCL. Moreover, they indicate heterogeneity in miR-155 expression among malignant T cells.

AB - Recently, miR-155 has been implicated in cutaneous T-cell lymphoma (CTCL). Thus, elevated levels of miR-155 were observed in skin lesions from CTCL patients as judged from qPCR and micro-array analysis and aberrant, high miR-155 expression was associated with severe disease. Moreover, miR-155 promoted proliferation of malignant T cells in vitro. Little is, however, known about which cell types express miR-155 in vivo in CTCL skin lesions. Here, we study miR-155 expression using in situ hybridization (ISH) with a miR-155 probe, a negative control (scrambled), and a miR-126 probe as a positive control in nine patients with mycosis fungoides, the most frequent subtype of CTCL. We provide evidence that both malignant and non-malignant T cells stain weakly to moderately positive with the miR-155 probe, but generally negative with the miR-126 and negative control probes. Reversely, endothelial cells stain positive for miR-126 and negative for miR-155 and the control probe. Solitary T cells with a malignant morphology display brighter staining with the miR-155 probe. Taken together, our findings suggest that both malignant and non-malignant T cells express miR-155 in situ in CTCL. Moreover, they indicate heterogeneity in miR-155 expression among malignant T cells.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Female

KW - Humans

KW - In Situ Hybridization

KW - Lymphoma, T-Cell, Cutaneous

KW - Male

KW - MicroRNAs

KW - Middle Aged

KW - Skin Neoplasms

U2 - 10.1111/apm.12162

DO - 10.1111/apm.12162

M3 - Journal article

C2 - 24033365

VL - 121

SP - 1020

EP - 1024

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 11

ER -

ID: 117551720