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Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model. / Rasmussen, Torben Sølbeck; Mentzel, Caroline Märta Junker; Kot, Witold; Castro-Mejiá, Josué Leonardo; Zuffa, Simone; Swann, Jonathan Richard; Hansen, Lars Hestbjerg; Vogensen, Finn Kvist; Hansen, Axel Kornerup; Nielsen, Dennis Sandris.

I: Gut, Bind 69, Nr. 12, 2020, s. 2122-2130.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Rasmussen, TS, Mentzel, CMJ, Kot, W, Castro-Mejiá, JL, Zuffa, S, Swann, JR, Hansen, LH, Vogensen, FK, Hansen, AK & Nielsen, DS 2020, 'Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model', Gut, bind 69, nr. 12, s. 2122-2130. https://doi.org/10.1136/gutjnl-2019-320005

APA

Rasmussen, T. S., Mentzel, C. M. J., Kot, W., Castro-Mejiá, J. L., Zuffa, S., Swann, J. R., Hansen, L. H., Vogensen, F. K., Hansen, A. K., & Nielsen, D. S. (2020). Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model. Gut, 69(12), 2122-2130. https://doi.org/10.1136/gutjnl-2019-320005

Vancouver

Rasmussen TS, Mentzel CMJ, Kot W, Castro-Mejiá JL, Zuffa S, Swann JR o.a. Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model. Gut. 2020;69(12):2122-2130. https://doi.org/10.1136/gutjnl-2019-320005

Author

Rasmussen, Torben Sølbeck ; Mentzel, Caroline Märta Junker ; Kot, Witold ; Castro-Mejiá, Josué Leonardo ; Zuffa, Simone ; Swann, Jonathan Richard ; Hansen, Lars Hestbjerg ; Vogensen, Finn Kvist ; Hansen, Axel Kornerup ; Nielsen, Dennis Sandris. / Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model. I: Gut. 2020 ; Bind 69, Nr. 12. s. 2122-2130.

Bibtex

@article{471fd18eb7c84b7fa2beb1ab5bec2402,
title = "Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model",
abstract = "Objective: Development of obesity and type 2 diabetes (T2D) are associated with gut microbiota (GM) changes. The gut viral community is predominated by bacteriophages (phages), which are viruses that attack bacteria in a host-specific manner. The antagonistic behaviour of phages has the potential to alter the GM. As a proof-of-concept, we demonstrate the efficacy of faecal virome transplantation (FVT) from lean donors for shifting the phenotype of obese mice into closer resemblance of lean mice. Design: The FVT consisted of viromes with distinct profiles extracted from the caecal content of mice from different vendors that were fed a low-fat (LF) diet for 14 weeks. Male C57BL/6NTac mice were divided into five groups: LF (as diet control), high-fat (HF) diet, HF+ampicillin (Amp), HF+Amp+FVT and HF+FVT. At weeks 6 and 7 of the study, the HF+FVT and HF+Amp+FVT mice were treated with FVT by oral gavage. The Amp groups were treated with Amp 24 hours prior to first FVT treatment. Results: Six weeks after first FVT, the HF+FVT mice showed a significant decrease in weight gain compared with the HF group. Further, glucose tolerance was comparable between the LF and HF+FVT mice, while the other HF groups all had impaired glucose tolerance. These observations were supported by significant shifts in GM composition, blood plasma metabolome and expression levels of genes associated with obesity and T2D development. Conclusions: Transfer of caecal viral communities from mice with a lean phenotype into mice with an obese phenotype led to reduced weight gain and normalised blood glucose parameters relative to lean mice. We hypothesise that this effect is mediated via FVT-induced GM changes.",
keywords = "diabetes mellitus, gut differentiation, intestinal microbiology, obesity",
author = "Rasmussen, {Torben S{\o}lbeck} and Mentzel, {Caroline M{\"a}rta Junker} and Witold Kot and Castro-Meji{\'a}, {Josu{\'e} Leonardo} and Simone Zuffa and Swann, {Jonathan Richard} and Hansen, {Lars Hestbjerg} and Vogensen, {Finn Kvist} and Hansen, {Axel Kornerup} and Nielsen, {Dennis Sandris}",
year = "2020",
doi = "10.1136/gutjnl-2019-320005",
language = "English",
volume = "69",
pages = "2122--2130",
journal = "Gut",
issn = "0017-5749",
publisher = "B M J Group",
number = "12",

}

RIS

TY - JOUR

T1 - Faecal virome transplantation decreases symptoms of type 2 diabetes and obesity in a murine model

AU - Rasmussen, Torben Sølbeck

AU - Mentzel, Caroline Märta Junker

AU - Kot, Witold

AU - Castro-Mejiá, Josué Leonardo

AU - Zuffa, Simone

AU - Swann, Jonathan Richard

AU - Hansen, Lars Hestbjerg

AU - Vogensen, Finn Kvist

AU - Hansen, Axel Kornerup

AU - Nielsen, Dennis Sandris

PY - 2020

Y1 - 2020

N2 - Objective: Development of obesity and type 2 diabetes (T2D) are associated with gut microbiota (GM) changes. The gut viral community is predominated by bacteriophages (phages), which are viruses that attack bacteria in a host-specific manner. The antagonistic behaviour of phages has the potential to alter the GM. As a proof-of-concept, we demonstrate the efficacy of faecal virome transplantation (FVT) from lean donors for shifting the phenotype of obese mice into closer resemblance of lean mice. Design: The FVT consisted of viromes with distinct profiles extracted from the caecal content of mice from different vendors that were fed a low-fat (LF) diet for 14 weeks. Male C57BL/6NTac mice were divided into five groups: LF (as diet control), high-fat (HF) diet, HF+ampicillin (Amp), HF+Amp+FVT and HF+FVT. At weeks 6 and 7 of the study, the HF+FVT and HF+Amp+FVT mice were treated with FVT by oral gavage. The Amp groups were treated with Amp 24 hours prior to first FVT treatment. Results: Six weeks after first FVT, the HF+FVT mice showed a significant decrease in weight gain compared with the HF group. Further, glucose tolerance was comparable between the LF and HF+FVT mice, while the other HF groups all had impaired glucose tolerance. These observations were supported by significant shifts in GM composition, blood plasma metabolome and expression levels of genes associated with obesity and T2D development. Conclusions: Transfer of caecal viral communities from mice with a lean phenotype into mice with an obese phenotype led to reduced weight gain and normalised blood glucose parameters relative to lean mice. We hypothesise that this effect is mediated via FVT-induced GM changes.

AB - Objective: Development of obesity and type 2 diabetes (T2D) are associated with gut microbiota (GM) changes. The gut viral community is predominated by bacteriophages (phages), which are viruses that attack bacteria in a host-specific manner. The antagonistic behaviour of phages has the potential to alter the GM. As a proof-of-concept, we demonstrate the efficacy of faecal virome transplantation (FVT) from lean donors for shifting the phenotype of obese mice into closer resemblance of lean mice. Design: The FVT consisted of viromes with distinct profiles extracted from the caecal content of mice from different vendors that were fed a low-fat (LF) diet for 14 weeks. Male C57BL/6NTac mice were divided into five groups: LF (as diet control), high-fat (HF) diet, HF+ampicillin (Amp), HF+Amp+FVT and HF+FVT. At weeks 6 and 7 of the study, the HF+FVT and HF+Amp+FVT mice were treated with FVT by oral gavage. The Amp groups were treated with Amp 24 hours prior to first FVT treatment. Results: Six weeks after first FVT, the HF+FVT mice showed a significant decrease in weight gain compared with the HF group. Further, glucose tolerance was comparable between the LF and HF+FVT mice, while the other HF groups all had impaired glucose tolerance. These observations were supported by significant shifts in GM composition, blood plasma metabolome and expression levels of genes associated with obesity and T2D development. Conclusions: Transfer of caecal viral communities from mice with a lean phenotype into mice with an obese phenotype led to reduced weight gain and normalised blood glucose parameters relative to lean mice. We hypothesise that this effect is mediated via FVT-induced GM changes.

KW - diabetes mellitus

KW - gut differentiation

KW - intestinal microbiology

KW - obesity

U2 - 10.1136/gutjnl-2019-320005

DO - 10.1136/gutjnl-2019-320005

M3 - Journal article

C2 - 32165408

AN - SCOPUS:85082531496

VL - 69

SP - 2122

EP - 2130

JO - Gut

JF - Gut

SN - 0017-5749

IS - 12

ER -

ID: 240141985