Forskning ved Københavns Universitet - Københavns Universitet

Forside

Family relationship of female breeders reduce the systematic inter-individual variation in the gut microbiota of inbred laboratory mice

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

The gut microbiota (GM) may influence disease expression in several animal models for inflammatory diseases. It may therefore seem reasonable to pursue reduction in the number of animals used for individual studies by reducing the variation in the GM. Previous studies have shown that the composition of the GM is related to genetics to a certain extent. We hypothesized that the GM similarity in a group of mice born by mothers not being sisters would be lower than that in a group born by mothers being sisters. The lower similarity could lead to clustering of the GM of mice born by non-sisters according to their mothers, while such clustering would not be visible if the mothers were sisters. We used 16S rRNA gene (V3 region) polymerase chain reaction-derived amplicon profiling by denaturing gradient gel electrophoresis (DGGE) to study the GM composition in caecum samples of 33 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were sisters, as well as caecum samples of 35 eight-week-old C57BL/6Sca mice from a breeding set-up with dam breeders that were not sisters. Principal component analysis revealed a significant difference between the litters from the breeding set-up with dam breeders that were not sisters, whereas no significant difference between the litters based on the breeding set-up with dam breeders that were sisters was observed. The results obtained indicate that the systematic variation in the GM of inbred mice can be reduced by increasing the family relatedness of the breeding pairs.

OriginalsprogEngelsk
TidsskriftLaboratory Animals. Journal of the Laboratory Animal Science Association
Vol/bind44
Udgave nummer4
Sider (fra-til)283-289
Antal sider7
ISSN0023-6772
DOI
StatusUdgivet - 2010

    Forskningsområder

  • Det tidligere LIFE - Laboratory animals, variation of the gut microbiota, Reduction, PCR/DGGE

ID: 32375174