Forskning ved Københavns Universitet - Københavns Universitet


Feeding mink (Neovison vison) a protein-restricted diet during pregnancy induces higher birth weight and altered hepatic gene expression in the F2 offspring

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Malnutrition during foetal life can induce modifications in the phenotype of an individual. The present study aimed to observe effects of low foetal life protein provision on modifications of the phenotype and changes in the progeny of 1-year-old female mink (F1 generation) offspring of mothers fed a low-protein diet. Traits studied included reproductive performance, energy and protein metabolism, and key hepatic enzymes associated with glucose homeostasis and metabolic hormones. The F0 generation offspring were fed either a low-protein (14 % of metabolisable energy (ME) from protein - FLP1) or an adequate-protein (29 % of ME from protein - FAP1) diet for the last 17.9 (sd 3.6) d of gestation. The F1 dams were studied at birth and at 1 year of age, during their first reproductive cycle, after maintenance on an adequate diet from birth and thereafter. Metabolic traits during gestation and lactation were largely unaffected by foetal life protein provision, but birth weight in the F2 generation was higher (P = 0.003) among FLP2 kits than among FAP2 kits. Furthermore, the relative abundance of pyruvate kinase mRNA was significantly (P = 0.007) lower, and fructose-1,6-bisphosphatase mRNA tended (P = 0.08) to be lower in FLP2 foetuses than in FAP2 foetuses, showing some similar difference in the F2 generation and F1 generation foetuses, suggesting an effect on some hepatic enzymes affecting glucose homeostasis being transmitted from the F1 to the F2 generation. These findings indicate that even though energy and nitrogen metabolism displayed no effect of protein provision during early life, programming effects still appeared at the molecular level in the following generation
TidsskriftBritish Journal of Nutrition
Udgave nummer4
Sider (fra-til)544-553
Antal sider10
StatusUdgivet - 2010

ID: 20293507