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FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis. / Ryder, L Rebekka; Bartels, Else Marie; Woetmann, Anders; Madsen, Hans Ole; Ødum, Niels; Bliddal, Henning; Danneskiold-Samsøe, Bente; Ribel-Madsen, Søren; Ryder, Lars P.

I: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Bind 120, Nr. 5, 05.2012, s. 387-396.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Ryder, LR, Bartels, EM, Woetmann, A, Madsen, HO, Ødum, N, Bliddal, H, Danneskiold-Samsøe, B, Ribel-Madsen, S & Ryder, LP 2012, 'FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis', APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, bind 120, nr. 5, s. 387-396. https://doi.org/10.1111/j.1600-0463.2011.02848.x

APA

Ryder, L. R., Bartels, E. M., Woetmann, A., Madsen, H. O., Ødum, N., Bliddal, H., ... Ryder, L. P. (2012). FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 120(5), 387-396. https://doi.org/10.1111/j.1600-0463.2011.02848.x

Vancouver

Ryder LR, Bartels EM, Woetmann A, Madsen HO, Ødum N, Bliddal H o.a. FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis. APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2012 maj;120(5):387-396. https://doi.org/10.1111/j.1600-0463.2011.02848.x

Author

Ryder, L Rebekka ; Bartels, Else Marie ; Woetmann, Anders ; Madsen, Hans Ole ; Ødum, Niels ; Bliddal, Henning ; Danneskiold-Samsøe, Bente ; Ribel-Madsen, Søren ; Ryder, Lars P. / FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis. I: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica. 2012 ; Bind 120, Nr. 5. s. 387-396.

Bibtex

@article{1b3b74016e4e448ea797276d2b3f8b3f,
title = "FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis",
abstract = "Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.",
keywords = "Adult, Aged, Aged, 80 and over, Alternative Splicing, Arthritis, Psoriatic, Arthritis, Rheumatoid, CD4-Positive T-Lymphocytes, Female, Forkhead Transcription Factors, Humans, Male, Middle Aged, Protein Isoforms, RNA, Messenger, Real-Time Polymerase Chain Reaction, Statistics, Nonparametric, Synovial Fluid, Young Adult",
author = "Ryder, {L Rebekka} and Bartels, {Else Marie} and Anders Woetmann and Madsen, {Hans Ole} and Niels {\O}dum and Henning Bliddal and Bente Danneskiold-Sams{\o}e and S{\o}ren Ribel-Madsen and Ryder, {Lars P}",
note = "{\circledC} 2011 The Authors. APMIS {\circledC} 2011 APMIS.",
year = "2012",
month = "5",
doi = "10.1111/j.1600-0463.2011.02848.x",
language = "English",
volume = "120",
pages = "387--396",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "5",

}

RIS

TY - JOUR

T1 - FoxP3 mRNA splice forms in synovial CD4+ T cells in rheumatoid arthritis and psoriatic arthritis

AU - Ryder, L Rebekka

AU - Bartels, Else Marie

AU - Woetmann, Anders

AU - Madsen, Hans Ole

AU - Ødum, Niels

AU - Bliddal, Henning

AU - Danneskiold-Samsøe, Bente

AU - Ribel-Madsen, Søren

AU - Ryder, Lars P

N1 - © 2011 The Authors. APMIS © 2011 APMIS.

PY - 2012/5

Y1 - 2012/5

N2 - Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.

AB - Our aim was to elucidate the relative amount of the different splice forms of FoxP3 mRNA in CD4+ T cells in peripheral blood (PB) compared to synovial fluid (SF) in RA and PsA patients. FoxP3 mRNA was measured using a quantitative real-time PCR method. CD4+ T cells were isolated from 17 paired samples of PB and SF from RA and PsA patients, and PB from 10 controls. FoxP3fl and FoxP3Δ2 mRNA was significantly increased (6.7 and 2.1-fold, respectively) in PB CD4+ T cells from RA patients compared to controls. FoxP3fl and Δ2 mRNA in SF CD4+ T cells was increased compared to controls in sero-negative RA and PsA, but not in sero-positive RA patients, who had a high FoxP3 expression in both PB and SF. The FoxP3Δ2Δ7 mRNA was barely detectable in patient samples, and not at all in healthy individuals. We provide evidence of an increased expression of FoxP3 splice forms in synovial CD4+ T cells from RA patients. A skewed, high expression profile of FoxP3, but not CTLA-4, in sero-negative RA and PsA, indicates that synovial CD4+ T cells may represent unique subsets of T cells which have been induced locally or selectively recruited to the joint.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Alternative Splicing

KW - Arthritis, Psoriatic

KW - Arthritis, Rheumatoid

KW - CD4-Positive T-Lymphocytes

KW - Female

KW - Forkhead Transcription Factors

KW - Humans

KW - Male

KW - Middle Aged

KW - Protein Isoforms

KW - RNA, Messenger

KW - Real-Time Polymerase Chain Reaction

KW - Statistics, Nonparametric

KW - Synovial Fluid

KW - Young Adult

U2 - 10.1111/j.1600-0463.2011.02848.x

DO - 10.1111/j.1600-0463.2011.02848.x

M3 - Journal article

C2 - 22515293

VL - 120

SP - 387

EP - 396

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 5

ER -

ID: 46486743