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Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells

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Standard

Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells. / Rubin, B; Geisler, C; Kuhlmann, J; Plesner, T.

I: Cellular Immunology, Bind 119, Nr. 2, 1989, s. 327-40.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Rubin, B, Geisler, C, Kuhlmann, J & Plesner, T 1989, 'Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells', Cellular Immunology, bind 119, nr. 2, s. 327-40.

APA

Rubin, B., Geisler, C., Kuhlmann, J., & Plesner, T. (1989). Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells. Cellular Immunology, 119(2), 327-40.

Vancouver

Rubin B, Geisler C, Kuhlmann J, Plesner T. Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells. Cellular Immunology. 1989;119(2):327-40.

Author

Rubin, B ; Geisler, C ; Kuhlmann, J ; Plesner, T. / Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells. I: Cellular Immunology. 1989 ; Bind 119, Nr. 2. s. 327-40.

Bibtex

@article{15c92bd0b0a911ddb538000ea68e967b,
title = "Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells",
abstract = "In the present experiments we have explored the possibilities of a modified immunoadsorbent technique to select for (1) mutagenized T cell receptor (Tcr) negative variants of Jurkat T lymphoma cells and (2) purified CD-4+ or CD-8+ T lymphocytes. The basic principle was to make large numbers of immunoglobulin (Ig) negative T cells Ig+ by T cell subset-specific monoclonal antibodies (mAb), and to select such cells on Ig anti-Ig columns. Our results demonstrated that Thy-1+, Fc receptor positive, antigen-specific T cells regulate the immune response in mice nonresponders to pork insulin, and the {"}autologous{"} mixed lymphocyte reaction. In addition, the immunoadsorbent method very efficiently selects Tcr/CD-3- variants from mutagenized Jurkat cell populations incubated with anti-CD3 mAb. The described method is easy and quick and can fractionate large numbers of cells; it is the {"}poor-man's cell sorter.{"} The most important finding is the demonstration of antigen-specific Thy-1+, CD-8+, and Fc receptor+ T suppressor cell that apparently react with antigen in a non-major histocompatibility complex-restricted manner.",
author = "B Rubin and C Geisler and J Kuhlmann and T Plesner",
note = "Keywords: Animals; Antigens; Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Cell Fractionation; Immunosorbent Techniques; Insulin; Lymphocyte Culture Test, Mixed; Mice; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; T-Lymphocytes; T-Lymphocytes, Helper-Inducer; T-Lymphocytes, Regulatory",
year = "1989",
language = "English",
volume = "119",
pages = "327--40",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press",
number = "2",

}

RIS

TY - JOUR

T1 - Fractionation of T cell subsets on Ig anti-Ig columns: isolation of helper T cells from nonresponder mice, demonstration of antigen-specific T suppressor cells, and selection of CD-3 negative variants of Jurkat T cells

AU - Rubin, B

AU - Geisler, C

AU - Kuhlmann, J

AU - Plesner, T

N1 - Keywords: Animals; Antigens; Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Cell Fractionation; Immunosorbent Techniques; Insulin; Lymphocyte Culture Test, Mixed; Mice; Mice, Inbred C57BL; Receptors, Antigen, T-Cell; T-Lymphocytes; T-Lymphocytes, Helper-Inducer; T-Lymphocytes, Regulatory

PY - 1989

Y1 - 1989

N2 - In the present experiments we have explored the possibilities of a modified immunoadsorbent technique to select for (1) mutagenized T cell receptor (Tcr) negative variants of Jurkat T lymphoma cells and (2) purified CD-4+ or CD-8+ T lymphocytes. The basic principle was to make large numbers of immunoglobulin (Ig) negative T cells Ig+ by T cell subset-specific monoclonal antibodies (mAb), and to select such cells on Ig anti-Ig columns. Our results demonstrated that Thy-1+, Fc receptor positive, antigen-specific T cells regulate the immune response in mice nonresponders to pork insulin, and the "autologous" mixed lymphocyte reaction. In addition, the immunoadsorbent method very efficiently selects Tcr/CD-3- variants from mutagenized Jurkat cell populations incubated with anti-CD3 mAb. The described method is easy and quick and can fractionate large numbers of cells; it is the "poor-man's cell sorter." The most important finding is the demonstration of antigen-specific Thy-1+, CD-8+, and Fc receptor+ T suppressor cell that apparently react with antigen in a non-major histocompatibility complex-restricted manner.

AB - In the present experiments we have explored the possibilities of a modified immunoadsorbent technique to select for (1) mutagenized T cell receptor (Tcr) negative variants of Jurkat T lymphoma cells and (2) purified CD-4+ or CD-8+ T lymphocytes. The basic principle was to make large numbers of immunoglobulin (Ig) negative T cells Ig+ by T cell subset-specific monoclonal antibodies (mAb), and to select such cells on Ig anti-Ig columns. Our results demonstrated that Thy-1+, Fc receptor positive, antigen-specific T cells regulate the immune response in mice nonresponders to pork insulin, and the "autologous" mixed lymphocyte reaction. In addition, the immunoadsorbent method very efficiently selects Tcr/CD-3- variants from mutagenized Jurkat cell populations incubated with anti-CD3 mAb. The described method is easy and quick and can fractionate large numbers of cells; it is the "poor-man's cell sorter." The most important finding is the demonstration of antigen-specific Thy-1+, CD-8+, and Fc receptor+ T suppressor cell that apparently react with antigen in a non-major histocompatibility complex-restricted manner.

M3 - Journal article

C2 - 2522821

VL - 119

SP - 327

EP - 340

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 2

ER -

ID: 8546581