Forskning ved Københavns Universitet - Københavns Universitet

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Full-length open reading frame amplification of hepatitis C virus

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

The purpose of this method is to amplify the full coding sequence of hepatitis C virus (HCV) by a single round reverse transcriptase-polymerase chain reaction (RT-PCR) approach. Our method relies on a highly robust and sensitive RNA extraction procedure and cutting-edge RT-PCR enzymes, all of which have been rigorously tested and optimized. This will not only allow for robust amplification of the entire open reading frame (ORF) of HCV for sequencing by Sanger or next-generation sequencing (NGS), but can also be used for cloning of the ORF of uncharacterized samples and for linkage analysis of mutations on individual genomes spanning the entire ORF. The method has been validated on a variety of samples, including sera from HCV patients and cell-culture supernatants.

OriginalsprogEngelsk
TitelHepatitis C Virus Protocols
Antal sider7
ForlagHumana Press
Publikationsdato2019
Sider85-91
ISBN (Trykt)978-1-4939-8975-1
ISBN (Elektronisk)978-1-4939-8976-8
DOI
StatusUdgivet - 2019
NavnMethods in Molecular Biology
Vol/bind1911
ISSN1064-3745

ID: 212851632