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Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing

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Standard

Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing. / Svenningsen, Sine L; Tu, Kimberly C; Bassler, Bonnie L.

I: EMBO Journal, Bind 28, Nr. 4, 2009, s. 429-39.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Svenningsen, SL, Tu, KC & Bassler, BL 2009, 'Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing', EMBO Journal, bind 28, nr. 4, s. 429-39. https://doi.org/10.1038/emboj.2008.300

APA

Svenningsen, S. L., Tu, K. C., & Bassler, B. L. (2009). Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing. EMBO Journal, 28(4), 429-39. https://doi.org/10.1038/emboj.2008.300

Vancouver

Svenningsen SL, Tu KC, Bassler BL. Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing. EMBO Journal. 2009;28(4):429-39. https://doi.org/10.1038/emboj.2008.300

Author

Svenningsen, Sine L ; Tu, Kimberly C ; Bassler, Bonnie L. / Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing. I: EMBO Journal. 2009 ; Bind 28, Nr. 4. s. 429-39.

Bibtex

@article{b284c1c0276111df8ed1000ea68e967b,
title = "Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing",
abstract = "Quorum sensing is a mechanism of cell-to-cell communication that allows bacteria to coordinately regulate gene expression in response to changes in cell-population density. At the core of the Vibrio cholerae quorum-sensing signal transduction pathway reside four homologous small RNAs (sRNAs), named the quorum regulatory RNAs 1-4 (Qrr1-4). The four Qrr sRNAs are functionally redundant. That is, expression of any one of them is sufficient for wild-type quorum-sensing behaviour. Here, we show that the combined action of two feedback loops, one involving the sRNA-activator LuxO and one involving the sRNA-target HapR, promotes gene dosage compensation between the four qrr genes. Gene dosage compensation adjusts the total Qrr1-4 sRNA pool and provides the molecular mechanism underlying sRNA redundancy. The dosage compensation mechanism is exquisitely sensitive to small perturbations in Qrr levels. Precisely maintained Qrr levels are required to direct the proper timing and correct patterns of expression of quorum-sensing-regulated target genes.",
author = "Svenningsen, {Sine L} and Tu, {Kimberly C} and Bassler, {Bonnie L}",
note = "Keywords: Bacterial Proteins; Base Sequence; Calibration; Dosage Compensation, Genetic; Flow Cytometry; Gene Deletion; Gene Expression Regulation, Bacterial; Models, Biological; Molecular Sequence Data; Mutation; Phosphoproteins; Quorum Sensing; RNA; Repressor Proteins; Sequence Homology, Nucleic Acid; Vibrio cholerae",
year = "2009",
doi = "10.1038/emboj.2008.300",
language = "English",
volume = "28",
pages = "429--39",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Gene dosage compensation calibrates four regulatory RNAs to control Vibrio cholerae quorum sensing

AU - Svenningsen, Sine L

AU - Tu, Kimberly C

AU - Bassler, Bonnie L

N1 - Keywords: Bacterial Proteins; Base Sequence; Calibration; Dosage Compensation, Genetic; Flow Cytometry; Gene Deletion; Gene Expression Regulation, Bacterial; Models, Biological; Molecular Sequence Data; Mutation; Phosphoproteins; Quorum Sensing; RNA; Repressor Proteins; Sequence Homology, Nucleic Acid; Vibrio cholerae

PY - 2009

Y1 - 2009

N2 - Quorum sensing is a mechanism of cell-to-cell communication that allows bacteria to coordinately regulate gene expression in response to changes in cell-population density. At the core of the Vibrio cholerae quorum-sensing signal transduction pathway reside four homologous small RNAs (sRNAs), named the quorum regulatory RNAs 1-4 (Qrr1-4). The four Qrr sRNAs are functionally redundant. That is, expression of any one of them is sufficient for wild-type quorum-sensing behaviour. Here, we show that the combined action of two feedback loops, one involving the sRNA-activator LuxO and one involving the sRNA-target HapR, promotes gene dosage compensation between the four qrr genes. Gene dosage compensation adjusts the total Qrr1-4 sRNA pool and provides the molecular mechanism underlying sRNA redundancy. The dosage compensation mechanism is exquisitely sensitive to small perturbations in Qrr levels. Precisely maintained Qrr levels are required to direct the proper timing and correct patterns of expression of quorum-sensing-regulated target genes.

AB - Quorum sensing is a mechanism of cell-to-cell communication that allows bacteria to coordinately regulate gene expression in response to changes in cell-population density. At the core of the Vibrio cholerae quorum-sensing signal transduction pathway reside four homologous small RNAs (sRNAs), named the quorum regulatory RNAs 1-4 (Qrr1-4). The four Qrr sRNAs are functionally redundant. That is, expression of any one of them is sufficient for wild-type quorum-sensing behaviour. Here, we show that the combined action of two feedback loops, one involving the sRNA-activator LuxO and one involving the sRNA-target HapR, promotes gene dosage compensation between the four qrr genes. Gene dosage compensation adjusts the total Qrr1-4 sRNA pool and provides the molecular mechanism underlying sRNA redundancy. The dosage compensation mechanism is exquisitely sensitive to small perturbations in Qrr levels. Precisely maintained Qrr levels are required to direct the proper timing and correct patterns of expression of quorum-sensing-regulated target genes.

U2 - 10.1038/emboj.2008.300

DO - 10.1038/emboj.2008.300

M3 - Journal article

C2 - 19165149

VL - 28

SP - 429

EP - 439

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 4

ER -

ID: 18363946