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Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1. / Li, Tong; Pires, Carlota; Nielsen, Troels Tolstrup; Waldemar, Gunhild; Hjermind, Lena Elisabeth; Nielsen, Jørgen Erik; Dinnyes, Andras; Holst, Bjørn; Hyttel, Poul; Freude, Kristine.

I: Stem Cell Research, Bind 16, Nr. 2, 03.2016, s. 334-337.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Li, T, Pires, C, Nielsen, TT, Waldemar, G, Hjermind, LE, Nielsen, JE, Dinnyes, A, Holst, B, Hyttel, P & Freude, K 2016, 'Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1', Stem Cell Research, bind 16, nr. 2, s. 334-337. https://doi.org/10.1016/j.scr.2016.01.001

APA

Li, T., Pires, C., Nielsen, T. T., Waldemar, G., Hjermind, L. E., Nielsen, J. E., Dinnyes, A., Holst, B., Hyttel, P., & Freude, K. (2016). Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1. Stem Cell Research, 16(2), 334-337. https://doi.org/10.1016/j.scr.2016.01.001

Vancouver

Li T, Pires C, Nielsen TT, Waldemar G, Hjermind LE, Nielsen JE o.a. Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1. Stem Cell Research. 2016 mar;16(2):334-337. https://doi.org/10.1016/j.scr.2016.01.001

Author

Li, Tong ; Pires, Carlota ; Nielsen, Troels Tolstrup ; Waldemar, Gunhild ; Hjermind, Lena Elisabeth ; Nielsen, Jørgen Erik ; Dinnyes, Andras ; Holst, Bjørn ; Hyttel, Poul ; Freude, Kristine. / Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1. I: Stem Cell Research. 2016 ; Bind 16, Nr. 2. s. 334-337.

Bibtex

@article{3757f8e4e9604d0b89c0aa7d65d0138e,
title = "Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1",
abstract = "Skin fibroblasts were obtained from a 46-year-old symptomatic man carrying a M146I mutation in the presenilin 1 gene (PSEN1), responsible for causing Alzheimer's disease (AD). Induced pluripotent stem cells (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. M146I-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported M146I-iPSCs line may be a useful resource for in vitro modeling of familial AD.",
keywords = "Alzheimer Disease, Cell Differentiation, Cells, Cultured, Cellular Reprogramming, Embryoid Bodies, Exons, Humans, Induced Pluripotent Stem Cells, Karyotype, Male, Microscopy, Fluorescence, Middle Aged, Polymorphism, Single Nucleotide, Presenilin-1, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Transcription Factors, Journal Article, Research Support, Non-U.S. Gov't",
author = "Tong Li and Carlota Pires and Nielsen, {Troels Tolstrup} and Gunhild Waldemar and Hjermind, {Lena Elisabeth} and Nielsen, {J{\o}rgen Erik} and Andras Dinnyes and Bj{\o}rn Holst and Poul Hyttel and Kristine Freude",
note = "Copyright {\textcopyright} 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.",
year = "2016",
month = mar,
doi = "10.1016/j.scr.2016.01.001",
language = "English",
volume = "16",
pages = "334--337",
journal = "Stem Cell Research",
issn = "1873-5061",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Generation of induced pluripotent stem cells (iPSCs) from an Alzheimer's disease patient carrying a M146I mutation in PSEN1

AU - Li, Tong

AU - Pires, Carlota

AU - Nielsen, Troels Tolstrup

AU - Waldemar, Gunhild

AU - Hjermind, Lena Elisabeth

AU - Nielsen, Jørgen Erik

AU - Dinnyes, Andras

AU - Holst, Bjørn

AU - Hyttel, Poul

AU - Freude, Kristine

N1 - Copyright © 2016 University of Texas at Austin Dell Medical School. Published by Elsevier B.V. All rights reserved.

PY - 2016/3

Y1 - 2016/3

N2 - Skin fibroblasts were obtained from a 46-year-old symptomatic man carrying a M146I mutation in the presenilin 1 gene (PSEN1), responsible for causing Alzheimer's disease (AD). Induced pluripotent stem cells (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. M146I-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported M146I-iPSCs line may be a useful resource for in vitro modeling of familial AD.

AB - Skin fibroblasts were obtained from a 46-year-old symptomatic man carrying a M146I mutation in the presenilin 1 gene (PSEN1), responsible for causing Alzheimer's disease (AD). Induced pluripotent stem cells (iPSCs) were derived via transfection with episomal vectors carrying hOCT4, hSOX2, hKLF2, hL-MYC, hLIN28 and shTP53 genes. M146I-iPSCs were free of genomically integrated reprogramming genes, had the specific mutation but no additional genomic aberrancies, expressed the expected pluripotency markers and displayed in vitro differentiation potential to the three germ layers. The reported M146I-iPSCs line may be a useful resource for in vitro modeling of familial AD.

KW - Alzheimer Disease

KW - Cell Differentiation

KW - Cells, Cultured

KW - Cellular Reprogramming

KW - Embryoid Bodies

KW - Exons

KW - Humans

KW - Induced Pluripotent Stem Cells

KW - Karyotype

KW - Male

KW - Microscopy, Fluorescence

KW - Middle Aged

KW - Polymorphism, Single Nucleotide

KW - Presenilin-1

KW - Real-Time Polymerase Chain Reaction

KW - Sequence Analysis, DNA

KW - Transcription Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.scr.2016.01.001

DO - 10.1016/j.scr.2016.01.001

M3 - Journal article

C2 - 27345998

VL - 16

SP - 334

EP - 337

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

IS - 2

ER -

ID: 172818329