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Genomic Analysis of Pasteurella atlantica Provides Insight on Its Virulence Factors and Phylogeny and Highlights the Potential of Reverse Vaccinology in Aquaculture

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Rebecca Marie Ellul
  • Panos G. Kalatzis
  • Cyril Frantzen
  • Gyri Teien Haugland
  • Snorre Gulla
  • Duncan John Colquhoun
  • Middelboe, Mathias
  • Heidrun Inger Wergeland
  • Anita Rønneseth

Pasteurellosis in farmed lumpsuckers, Cyclopterus lumpus, has emerged as a serious disease in Norwegian aquaculture in recent years. Genomic characterization of the causative agent is essential in understanding the biology of the bacteria involved and in devising an efficient preventive strategy. The genomes of two clinical Pasteurella atlantica isolates were sequenced (≈2.3 Mbp), and phylogenetic analysis confirmed their position as a novel species within the Pasteurellaceae. In silico analyses revealed 11 genomic islands and 5 prophages, highlighting the potential of mobile elements as driving forces in the evolution of this species. The previously documented pathogenicity of P. atlantica is strongly supported by the current study, and 17 target genes were recognized as putative primary drivers of pathogenicity. The expression level of a predicted vaccine target, an uncharacterized adhesin protein, was significantly increased in both broth culture and following the exposure of P. atlantica to lumpsucker head kidney leucocytes. Based on in silico and functional analyses, the strongest gene target candidates will be prioritized in future vaccine development efforts to prevent future pasteurellosis outbreaks.

OriginalsprogEngelsk
Artikelnummer1215
TidsskriftMicroorganisms
Vol/bind9
Udgave nummer6
Antal sider22
ISSN2076-2607
DOI
StatusUdgivet - 2021

Bibliografisk note

Funding Information:
This research was funded by the University of Bergen and supported by ACD Pharmaceuticals and the Norwegian Veterinary Institute. R.M.E. thanks Harald S?b? Lunde for his technical support during the design of the primers for the functional analyses in this work. PacBio sequencing was performed at the Norwegian Sequencing Centre (NSC) at the Department of Medical Genetics (AMG), Oslo University Hospital.

Funding Information:
Funding: This research was funded by the University of Bergen and supported by ACD Pharmaceuticals and the Norwegian Veterinary Institute.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).

ID: 280118850