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Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C

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Standard

Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. / Carlsson, Robert; Hjalmarsson, Anna; Liberg, David; Persson, Christine; Leanderson, Tomas.

I: Gene, Bind 299, Nr. 1-2, 2002, s. 271-8.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Carlsson, R, Hjalmarsson, A, Liberg, D, Persson, C & Leanderson, T 2002, 'Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C', Gene, bind 299, nr. 1-2, s. 271-8.

APA

Carlsson, R., Hjalmarsson, A., Liberg, D., Persson, C., & Leanderson, T. (2002). Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. Gene, 299(1-2), 271-8.

Vancouver

Carlsson R, Hjalmarsson A, Liberg D, Persson C, Leanderson T. Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. Gene. 2002;299(1-2):271-8.

Author

Carlsson, Robert ; Hjalmarsson, Anna ; Liberg, David ; Persson, Christine ; Leanderson, Tomas. / Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C. I: Gene. 2002 ; Bind 299, Nr. 1-2. s. 271-8.

Bibtex

@article{118c0a90e5a111dfb6d2000ea68e967b,
title = "Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C",
abstract = "Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5'-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single processed transcript. We have also isolated a cDNA clone encoding the human SPI-C homologue, which displays 65{\%} amino acid identity to the murine protein. In addition, we show that the genomic structure of the human and mouse genes are similar, containing a 5' non-coding exon followed by five coding exons. Human SPI-C mRNA is preferentially detected in foetal and adult spleen, lymph nodes and at lower levels in bone marrow and foetal liver. Finally a phylogenetic prediction analysis of SPI-group protein sequences suggest that the SPI-C proteins form a distinct subgroup, with human SPI-C being closest related to the mouse SPI-C protein.",
author = "Robert Carlsson and Anna Hjalmarsson and David Liberg and Christine Persson and Tomas Leanderson",
note = "Keywords: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary; DNA-Binding Proteins; Exons; Gene Expression Profiling; Genes; Hela Cells; Humans; Introns; K562 Cells; Mice; Molecular Sequence Data; Phylogeny; RNA, Messenger; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Transcription, Genetic; Tumor Cells, Cultured",
year = "2002",
language = "English",
volume = "299",
pages = "271--8",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Genomic structure of mouse SPI-C and genomic structure and expression pattern of human SPI-C

AU - Carlsson, Robert

AU - Hjalmarsson, Anna

AU - Liberg, David

AU - Persson, Christine

AU - Leanderson, Tomas

N1 - Keywords: Amino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; DNA, Complementary; DNA-Binding Proteins; Exons; Gene Expression Profiling; Genes; Hela Cells; Humans; Introns; K562 Cells; Mice; Molecular Sequence Data; Phylogeny; RNA, Messenger; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Transcription, Genetic; Tumor Cells, Cultured

PY - 2002

Y1 - 2002

N2 - Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5'-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single processed transcript. We have also isolated a cDNA clone encoding the human SPI-C homologue, which displays 65% amino acid identity to the murine protein. In addition, we show that the genomic structure of the human and mouse genes are similar, containing a 5' non-coding exon followed by five coding exons. Human SPI-C mRNA is preferentially detected in foetal and adult spleen, lymph nodes and at lower levels in bone marrow and foetal liver. Finally a phylogenetic prediction analysis of SPI-group protein sequences suggest that the SPI-C proteins form a distinct subgroup, with human SPI-C being closest related to the mouse SPI-C protein.

AB - Erythroblast transformation-specific domain (ETS) transcription factors regulate some of the critical molecular mechanisms controlling the differentiation of multipotent haematopoietic progenitor cells into effector B-lymphocytes. The SPI-group ETS-protein transcription factors PU.1 and SPI-B play essential and, although coexpressed and binding to similar DNA sequences, unique roles in B-cell differentiation in mice. Mouse SPI-C is an SPI-group ETS protein expressed temporarily during B-cell development and in macrophages. Here we present the genomic organization of the mouse SPI-C gene, and show by rapid amplification of cDNA ends (5'-RACE) analysis that transcription of the mouse SPI-C mRNA starts at a single site producing a single processed transcript. We have also isolated a cDNA clone encoding the human SPI-C homologue, which displays 65% amino acid identity to the murine protein. In addition, we show that the genomic structure of the human and mouse genes are similar, containing a 5' non-coding exon followed by five coding exons. Human SPI-C mRNA is preferentially detected in foetal and adult spleen, lymph nodes and at lower levels in bone marrow and foetal liver. Finally a phylogenetic prediction analysis of SPI-group protein sequences suggest that the SPI-C proteins form a distinct subgroup, with human SPI-C being closest related to the mouse SPI-C protein.

M3 - Journal article

C2 - 12459275

VL - 299

SP - 271

EP - 278

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -

ID: 22861458