Forskning ved Københavns Universitet - Københavns Universitet

Forside

gfp-based N-acyl homoserine-lactone sensor systems for detection of bacterial communication

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • Andersen, Jens Bo
  • Arne Heydorn
  • Morten Hentzer
  • Leo Eberl
  • O Geisenberger
  • B B Christensen
  • Søren Molin
  • M Givskov
In order to perform single-cell analysis and online studies of N-acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P(luxI) have been fused to modified versions of gfpmut3* genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N-3-oxohexanoyl-L-homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.
OriginalsprogEngelsk
TidsskriftApplied and Environmental Microbiology
Vol/bind67
Udgave nummer2
Sider (fra-til)575-85
Antal sider11
ISSN0099-2240
DOI
StatusUdgivet - 2001
Eksternt udgivetJa

ID: 46842459