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GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1

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Standard

GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1. / Guan, Xinfu; Stoll, Barbara; Lu, Xiaofeng; Tappenden, Kelly A; Holst, Jens Juul; Hartmann, Bolette; Burrin, Douglas G.

I: Gastroenterology, Bind 125, Nr. 1, 07.2003, s. 136-47.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Guan, X, Stoll, B, Lu, X, Tappenden, KA, Holst, JJ, Hartmann, B & Burrin, DG 2003, 'GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1', Gastroenterology, bind 125, nr. 1, s. 136-47.

APA

Guan, X., Stoll, B., Lu, X., Tappenden, K. A., Holst, J. J., Hartmann, B., & Burrin, D. G. (2003). GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1. Gastroenterology, 125(1), 136-47.

Vancouver

Guan X, Stoll B, Lu X, Tappenden KA, Holst JJ, Hartmann B o.a. GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1. Gastroenterology. 2003 jul;125(1):136-47.

Author

Guan, Xinfu ; Stoll, Barbara ; Lu, Xiaofeng ; Tappenden, Kelly A ; Holst, Jens Juul ; Hartmann, Bolette ; Burrin, Douglas G. / GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1. I: Gastroenterology. 2003 ; Bind 125, Nr. 1. s. 136-47.

Bibtex

@article{7760031c045c443fb610f2bbdee22fbb,
title = "GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1",
abstract = "BACKGROUND & AIMS: Our aim was to determine whether the intestinotrophic effects of GLP-2 are mediated by acute up-regulation of intestinal substrate utilization in TPN-fed piglets.METHODS: Twenty-four 12-day-old pigs, fitted with a portal flow probe and carotid, jugular and portal catheters, were fed by TPN for 7 days. On day 8, a group of pigs (n = 8) was infused intravenously with saline (control) for 4 hours and then with GLP-2 (500 pmol x kg(-1) x hour(-1), GLP-2) for 4 hours. (2)H-glucose and (13)C-phenylalanine were infused to estimate their kinetics and protein turnover. Another group (n = 8) received consecutive intravenous infusions of saline, GLP-2, and GLP-2 plus N(G)-Nitro-L-arginine methyl ester (L-NAME, 50 micromol x kg(-1) x hour(-1)) for 4 hours each.RESULTS: GLP-2 acutely increased portal-drained visceral (PDV) blood flow rate (+25{\%}) and intestinal blood volume (+51{\%}) in TPN-fed piglets. GLP-2 also increased intestinal constitutive nitric oxide synthase (NOS) activity and endothelial NOS protein abundance. GLP-2 acutely increased PDV glucose uptake (+90{\%}) and net lactate production (+79{\%}). Co-infusion of GLP-2 plus L-NAME did not increase either PDV blood flow rate or glucose uptake. GLP-2 increased PDV indispensable amino acid uptake by 220{\%} and protein synthesis by 125{\%}, but did not decrease protein breakdown or phenylalanine oxidation.CONCLUSIONS: We conclude that in TPN-fed neonatal pigs, GLP-2 acutely stimulates intestinal blood flow and glucose utilization, and this response is nitric oxide-dependent. These findings suggest that GLP-2 may play an important physiological role in the regulation of intestinal blood flow and that nitric oxide is involved in GLP-2 receptor function.",
keywords = "Amino Acids, Animals, Animals, Newborn, Dietary Proteins, Female, Glucagon-Like Peptide 2, Glucagon-Like Peptides, Glucose, Intestines, Liver Circulation, Nitric Oxide, Nitric Oxide Synthase, Oxygen Consumption, Pancreatic Hormones, Parenteral Nutrition, Total, Peptides, Portal System, Regional Blood Flow, Sus scrofa, Up-Regulation",
author = "Xinfu Guan and Barbara Stoll and Xiaofeng Lu and Tappenden, {Kelly A} and Holst, {Jens Juul} and Bolette Hartmann and Burrin, {Douglas G}",
year = "2003",
month = "7",
language = "English",
volume = "125",
pages = "136--47",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - GLP-2-mediated up-regulation of intestinal blood flow and glucose uptake is nitric oxide-dependent in TPN-fed piglets 1

AU - Guan, Xinfu

AU - Stoll, Barbara

AU - Lu, Xiaofeng

AU - Tappenden, Kelly A

AU - Holst, Jens Juul

AU - Hartmann, Bolette

AU - Burrin, Douglas G

PY - 2003/7

Y1 - 2003/7

N2 - BACKGROUND & AIMS: Our aim was to determine whether the intestinotrophic effects of GLP-2 are mediated by acute up-regulation of intestinal substrate utilization in TPN-fed piglets.METHODS: Twenty-four 12-day-old pigs, fitted with a portal flow probe and carotid, jugular and portal catheters, were fed by TPN for 7 days. On day 8, a group of pigs (n = 8) was infused intravenously with saline (control) for 4 hours and then with GLP-2 (500 pmol x kg(-1) x hour(-1), GLP-2) for 4 hours. (2)H-glucose and (13)C-phenylalanine were infused to estimate their kinetics and protein turnover. Another group (n = 8) received consecutive intravenous infusions of saline, GLP-2, and GLP-2 plus N(G)-Nitro-L-arginine methyl ester (L-NAME, 50 micromol x kg(-1) x hour(-1)) for 4 hours each.RESULTS: GLP-2 acutely increased portal-drained visceral (PDV) blood flow rate (+25%) and intestinal blood volume (+51%) in TPN-fed piglets. GLP-2 also increased intestinal constitutive nitric oxide synthase (NOS) activity and endothelial NOS protein abundance. GLP-2 acutely increased PDV glucose uptake (+90%) and net lactate production (+79%). Co-infusion of GLP-2 plus L-NAME did not increase either PDV blood flow rate or glucose uptake. GLP-2 increased PDV indispensable amino acid uptake by 220% and protein synthesis by 125%, but did not decrease protein breakdown or phenylalanine oxidation.CONCLUSIONS: We conclude that in TPN-fed neonatal pigs, GLP-2 acutely stimulates intestinal blood flow and glucose utilization, and this response is nitric oxide-dependent. These findings suggest that GLP-2 may play an important physiological role in the regulation of intestinal blood flow and that nitric oxide is involved in GLP-2 receptor function.

AB - BACKGROUND & AIMS: Our aim was to determine whether the intestinotrophic effects of GLP-2 are mediated by acute up-regulation of intestinal substrate utilization in TPN-fed piglets.METHODS: Twenty-four 12-day-old pigs, fitted with a portal flow probe and carotid, jugular and portal catheters, were fed by TPN for 7 days. On day 8, a group of pigs (n = 8) was infused intravenously with saline (control) for 4 hours and then with GLP-2 (500 pmol x kg(-1) x hour(-1), GLP-2) for 4 hours. (2)H-glucose and (13)C-phenylalanine were infused to estimate their kinetics and protein turnover. Another group (n = 8) received consecutive intravenous infusions of saline, GLP-2, and GLP-2 plus N(G)-Nitro-L-arginine methyl ester (L-NAME, 50 micromol x kg(-1) x hour(-1)) for 4 hours each.RESULTS: GLP-2 acutely increased portal-drained visceral (PDV) blood flow rate (+25%) and intestinal blood volume (+51%) in TPN-fed piglets. GLP-2 also increased intestinal constitutive nitric oxide synthase (NOS) activity and endothelial NOS protein abundance. GLP-2 acutely increased PDV glucose uptake (+90%) and net lactate production (+79%). Co-infusion of GLP-2 plus L-NAME did not increase either PDV blood flow rate or glucose uptake. GLP-2 increased PDV indispensable amino acid uptake by 220% and protein synthesis by 125%, but did not decrease protein breakdown or phenylalanine oxidation.CONCLUSIONS: We conclude that in TPN-fed neonatal pigs, GLP-2 acutely stimulates intestinal blood flow and glucose utilization, and this response is nitric oxide-dependent. These findings suggest that GLP-2 may play an important physiological role in the regulation of intestinal blood flow and that nitric oxide is involved in GLP-2 receptor function.

KW - Amino Acids

KW - Animals

KW - Animals, Newborn

KW - Dietary Proteins

KW - Female

KW - Glucagon-Like Peptide 2

KW - Glucagon-Like Peptides

KW - Glucose

KW - Intestines

KW - Liver Circulation

KW - Nitric Oxide

KW - Nitric Oxide Synthase

KW - Oxygen Consumption

KW - Pancreatic Hormones

KW - Parenteral Nutrition, Total

KW - Peptides

KW - Portal System

KW - Regional Blood Flow

KW - Sus scrofa

KW - Up-Regulation

M3 - Journal article

C2 - 12851879

VL - 125

SP - 136

EP - 147

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 1

ER -

ID: 132055938