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High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture

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  • Yun-Ji Shin
  • Shin-Young Hong
  • Tae-Ho Kwon
  • Yong-Suk Jang
  • Moon-Sik Yang

Recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) has been previously produced in tobacco cell suspension cultures. However, the amount of hGM-CSF accumulated in the culture medium dropped quickly from its maximum of 150 microg/L at 5 d after incubation. To overcome this problem, we sought an expression system in which heterologous gene expression could be induced at high levels. We selected a rice amylase expression system in which the promoter Ramy3D is induced to express recombinant protein by sucrose starvation. This induction system was found to give good yield of recombinant hGM-CSF in transgenic rice cell suspension culture and protease activity of this culture medium was low compared to that of tobacco culture system.

OriginalsprogEngelsk
TidsskriftBiotechnology and Bioengineering (Print)
Vol/bind82
Udgave nummer7
Sider (fra-til)778-83
Antal sider6
ISSN0006-3592
DOI
StatusUdgivet - 30 jun. 2003

ID: 162127945