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High-performance liquid chromatography on-line coupled to high-field NMR and mass spectrometry for structure elucidation of constituents of Hypericum perforatum L

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • S. H. Hansen
  • A. G. Jensen
  • Cornett, Claus
  • I. Bjornsdottir
  • S. Taylor
  • B. Wright
  • I. D. Wilson
The on-line separation and structure elucidation of naphthodianthrones, flavonoids, and other constituents of an extract from Hypericum perforatum L, using high performance liquid chromatography (HPLC) coupled on-line with ultraviolet-visible, nuclear magnetic resonance (NMR), and mass spectrometry (MS) is described. A conventional reversed-phase HPLC system using ammonium acetate as the buffer substance in the eluent tvas used, and proton NMR spectra were obtained on a 500 MHz NMR instrument. The MS and MS/MS analyses were performed using negative electrospray ionization, In the present study, all of the major known constituents in extracts from Hypericum perforatum L, were identified, and two new substances which had not previously been reported as constituents of extracts of Hypericum perforatum L. were identified and their structures elucidated.
OriginalsprogUdefineret/Ukendt
TidsskriftAnalytical Chemistry
Vol/bind71
Udgave nummer22
Sider (fra-til)5235-5241
Antal sider7
ISSN0003-2700
StatusUdgivet - 1999

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