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High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach

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High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. / Mollerup, Sarah; Asplund, Maria; Friis-Nielsen, Jens; Kjartansdóttir, Kristín Rós; Fridholm, Helena; Hansen, Thomas Arn; Herrera, José Alejandro Romero; Barnes, Christopher James; Jensen, Randi Holm; Richter, Stine Raith; Nielsen, Ida Broman; Pietroni, Carlotta; Alquezar-Planas, David E; Rey-Iglesia, Alba; Olsen, Pernille V S; Rajpert-De Meyts, Ewa; Groth-Pedersen, Line; von Buchwald, Christian; Jensen, David H; Gniadecki, Robert; Høgdall, Estrid; Langhoff, Jill Levin; Pete, Imre; Vereczkey, Ildikó; Baranyai, Zsolt; Dybkaer, Karen; Johnsen, Hans Erik; Steiniche, Torben; Hokland, Peter; Rosenberg, Jacob; Baandrup, Ulrik; Sicheritz-Pontén, Thomas; Willerslev, Eske; Brunak, Søren; Lund, Ole; Mourier, Tobias; Vinner, Lasse; Izarzugaza, Jose M G; Nielsen, Lars Peter; Hansen, Anders Johannes.

I: The Journal of Infectious Diseases, Bind 220, Nr. 8, 15.10.2019, s. 1312-1324.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Mollerup, S, Asplund, M, Friis-Nielsen, J, Kjartansdóttir, KR, Fridholm, H, Hansen, TA, Herrera, JAR, Barnes, CJ, Jensen, RH, Richter, SR, Nielsen, IB, Pietroni, C, Alquezar-Planas, DE, Rey-Iglesia, A, Olsen, PVS, Rajpert-De Meyts, E, Groth-Pedersen, L, von Buchwald, C, Jensen, DH, Gniadecki, R, Høgdall, E, Langhoff, JL, Pete, I, Vereczkey, I, Baranyai, Z, Dybkaer, K, Johnsen, HE, Steiniche, T, Hokland, P, Rosenberg, J, Baandrup, U, Sicheritz-Pontén, T, Willerslev, E, Brunak, S, Lund, O, Mourier, T, Vinner, L, Izarzugaza, JMG, Nielsen, LP & Hansen, AJ 2019, 'High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach', The Journal of Infectious Diseases, bind 220, nr. 8, s. 1312-1324. https://doi.org/10.1093/infdis/jiz318

APA

Mollerup, S., Asplund, M., Friis-Nielsen, J., Kjartansdóttir, K. R., Fridholm, H., Hansen, T. A., ... Hansen, A. J. (2019). High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. The Journal of Infectious Diseases, 220(8), 1312-1324. https://doi.org/10.1093/infdis/jiz318

Vancouver

Mollerup S, Asplund M, Friis-Nielsen J, Kjartansdóttir KR, Fridholm H, Hansen TA o.a. High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. The Journal of Infectious Diseases. 2019 okt 15;220(8):1312-1324. https://doi.org/10.1093/infdis/jiz318

Author

Mollerup, Sarah ; Asplund, Maria ; Friis-Nielsen, Jens ; Kjartansdóttir, Kristín Rós ; Fridholm, Helena ; Hansen, Thomas Arn ; Herrera, José Alejandro Romero ; Barnes, Christopher James ; Jensen, Randi Holm ; Richter, Stine Raith ; Nielsen, Ida Broman ; Pietroni, Carlotta ; Alquezar-Planas, David E ; Rey-Iglesia, Alba ; Olsen, Pernille V S ; Rajpert-De Meyts, Ewa ; Groth-Pedersen, Line ; von Buchwald, Christian ; Jensen, David H ; Gniadecki, Robert ; Høgdall, Estrid ; Langhoff, Jill Levin ; Pete, Imre ; Vereczkey, Ildikó ; Baranyai, Zsolt ; Dybkaer, Karen ; Johnsen, Hans Erik ; Steiniche, Torben ; Hokland, Peter ; Rosenberg, Jacob ; Baandrup, Ulrik ; Sicheritz-Pontén, Thomas ; Willerslev, Eske ; Brunak, Søren ; Lund, Ole ; Mourier, Tobias ; Vinner, Lasse ; Izarzugaza, Jose M G ; Nielsen, Lars Peter ; Hansen, Anders Johannes. / High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach. I: The Journal of Infectious Diseases. 2019 ; Bind 220, Nr. 8. s. 1312-1324.

Bibtex

@article{c48f709ff48a4edd80bee3e4e62ff20d,
title = "High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach",
abstract = "BACKGROUND: Viruses and other infectious agents cause more than 15{\%} of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.",
author = "Sarah Mollerup and Maria Asplund and Jens Friis-Nielsen and Kjartansd{\'o}ttir, {Krist{\'i}n R{\'o}s} and Helena Fridholm and Hansen, {Thomas Arn} and Herrera, {Jos{\'e} Alejandro Romero} and Barnes, {Christopher James} and Jensen, {Randi Holm} and Richter, {Stine Raith} and Nielsen, {Ida Broman} and Carlotta Pietroni and Alquezar-Planas, {David E} and Alba Rey-Iglesia and Olsen, {Pernille V S} and {Rajpert-De Meyts}, Ewa and Line Groth-Pedersen and {von Buchwald}, Christian and Jensen, {David H} and Robert Gniadecki and Estrid H{\o}gdall and Langhoff, {Jill Levin} and Imre Pete and Ildik{\'o} Vereczkey and Zsolt Baranyai and Karen Dybkaer and Johnsen, {Hans Erik} and Torben Steiniche and Peter Hokland and Jacob Rosenberg and Ulrik Baandrup and Thomas Sicheritz-Pont{\'e}n and Eske Willerslev and S{\o}ren Brunak and Ole Lund and Tobias Mourier and Lasse Vinner and Izarzugaza, {Jose M G} and Nielsen, {Lars Peter} and Hansen, {Anders Johannes}",
note = "{\circledC} The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.",
year = "2019",
month = "10",
day = "15",
doi = "10.1093/infdis/jiz318",
language = "English",
volume = "220",
pages = "1312--1324",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "8",

}

RIS

TY - JOUR

T1 - High-Throughput Sequencing-Based Investigation of Viruses in Human Cancers by Multienrichment Approach

AU - Mollerup, Sarah

AU - Asplund, Maria

AU - Friis-Nielsen, Jens

AU - Kjartansdóttir, Kristín Rós

AU - Fridholm, Helena

AU - Hansen, Thomas Arn

AU - Herrera, José Alejandro Romero

AU - Barnes, Christopher James

AU - Jensen, Randi Holm

AU - Richter, Stine Raith

AU - Nielsen, Ida Broman

AU - Pietroni, Carlotta

AU - Alquezar-Planas, David E

AU - Rey-Iglesia, Alba

AU - Olsen, Pernille V S

AU - Rajpert-De Meyts, Ewa

AU - Groth-Pedersen, Line

AU - von Buchwald, Christian

AU - Jensen, David H

AU - Gniadecki, Robert

AU - Høgdall, Estrid

AU - Langhoff, Jill Levin

AU - Pete, Imre

AU - Vereczkey, Ildikó

AU - Baranyai, Zsolt

AU - Dybkaer, Karen

AU - Johnsen, Hans Erik

AU - Steiniche, Torben

AU - Hokland, Peter

AU - Rosenberg, Jacob

AU - Baandrup, Ulrik

AU - Sicheritz-Pontén, Thomas

AU - Willerslev, Eske

AU - Brunak, Søren

AU - Lund, Ole

AU - Mourier, Tobias

AU - Vinner, Lasse

AU - Izarzugaza, Jose M G

AU - Nielsen, Lars Peter

AU - Hansen, Anders Johannes

N1 - © The Author(s) 2019. Published by Oxford University Press for the Infectious Diseases Society of America.

PY - 2019/10/15

Y1 - 2019/10/15

N2 - BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.

AB - BACKGROUND: Viruses and other infectious agents cause more than 15% of human cancer cases. High-throughput sequencing-based studies of virus-cancer associations have mainly focused on cancer transcriptome data.METHODS: In this study, we applied a diverse selection of presequencing enrichment methods targeting all major viral groups, to characterize the viruses present in 197 samples from 18 sample types of cancerous origin. Using high-throughput sequencing, we generated 710 datasets constituting 57 billion sequencing reads.RESULTS: Detailed in silico investigation of the viral content, including exclusion of viral artefacts, from de novo assembled contigs and individual sequencing reads yielded a map of the viruses detected. Our data reveal a virome dominated by papillomaviruses, anelloviruses, herpesviruses, and parvoviruses. More than half of the included samples contained 1 or more viruses; however, no link between specific viruses and cancer types were found.CONCLUSIONS: Our study sheds light on viral presence in cancers and provides highly relevant virome data for future reference.

U2 - 10.1093/infdis/jiz318

DO - 10.1093/infdis/jiz318

M3 - Journal article

VL - 220

SP - 1312

EP - 1324

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 8

ER -

ID: 227688808