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Human thymic epithelial cells present superantigens to T-cell lines and thymocytes

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Standard

Human thymic epithelial cells present superantigens to T-cell lines and thymocytes. / Jlrgensen, A; Nielsen, M; Svejgaard, A; Ledbetter, J A; Odum, Niels; Röpke, C.

I: Experimental and Clinical Immunogenetics, Bind 13, Nr. 3-4, 1996, s. 192-203.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Jlrgensen, A, Nielsen, M, Svejgaard, A, Ledbetter, JA, Odum, N & Röpke, C 1996, 'Human thymic epithelial cells present superantigens to T-cell lines and thymocytes', Experimental and Clinical Immunogenetics, bind 13, nr. 3-4, s. 192-203.

APA

Jlrgensen, A., Nielsen, M., Svejgaard, A., Ledbetter, J. A., Odum, N., & Röpke, C. (1996). Human thymic epithelial cells present superantigens to T-cell lines and thymocytes. Experimental and Clinical Immunogenetics, 13(3-4), 192-203.

Vancouver

Jlrgensen A, Nielsen M, Svejgaard A, Ledbetter JA, Odum N, Röpke C. Human thymic epithelial cells present superantigens to T-cell lines and thymocytes. Experimental and Clinical Immunogenetics. 1996;13(3-4):192-203.

Author

Jlrgensen, A ; Nielsen, M ; Svejgaard, A ; Ledbetter, J A ; Odum, Niels ; Röpke, C. / Human thymic epithelial cells present superantigens to T-cell lines and thymocytes. I: Experimental and Clinical Immunogenetics. 1996 ; Bind 13, Nr. 3-4. s. 192-203.

Bibtex

@article{afda7660fd9411ddb219000ea68e967b,
title = "Human thymic epithelial cells present superantigens to T-cell lines and thymocytes",
abstract = "It is generally accepted that thymic epithelial cells (TEC) act as accessory cells in positive selection of pre-T cells. However, our knowledge of the antigen presentation and accessory cell function to human TEC is limited. Here we present results obtained by the use of serum-free cultured human TEC, showing that IFN-gamma-treated TEC are able to support T-cell-mediated responses to the bacterial superantigens (Sag) SEA and SEB, even at very low Sag concentrations. T-cell responses to TEC-presented Sags were dependent on the presence of the adhesion molecules ICAM-1, ICAM-2, LFA-1, and LFA-3, but not on CD4 and CD8 molecules. There is a low but significant expression of B7 molecules on human TEC, and treatment of TEC with anti-B7.1 and anti-B7.2 antibodies before Sag pulsing leads to decreased Sag responses, indicating a significant importance of B7 molecules on TEC. Both CD4+ T-cell lines and CD4+ as well as CD8+ subpopulations of thymocytes showed significant responses, whereas nonseparated thymocytes, CD4+8+, and CD4-CD8- thymocytes did not respond or showed very low responses. In conclusion, the present results demonstrate that cultured human TEC are able to present Sag to thymocytes.",
author = "A Jlrgensen and M Nielsen and A Svejgaard and Ledbetter, {J A} and Niels Odum and C R{\"o}pke",
note = "Keywords: Antibodies; Antigen Presentation; Antigens, Bacterial; Antigens, CD80; CD4-Positive T-Lymphocytes; Cell Adhesion Molecules; Cell Count; Cell Line; Child, Preschool; Epithelial Cells; Epithelium; Humans; Infant; Superantigens; T-Lymphocyte Subsets; T-Lymphocytes; Thymus Gland",
year = "1996",
language = "English",
volume = "13",
pages = "192--203",
journal = "Experimental and Clinical Immunogenetics",
issn = "0254-9670",
publisher = "S Karger AG",
number = "3-4",

}

RIS

TY - JOUR

T1 - Human thymic epithelial cells present superantigens to T-cell lines and thymocytes

AU - Jlrgensen, A

AU - Nielsen, M

AU - Svejgaard, A

AU - Ledbetter, J A

AU - Odum, Niels

AU - Röpke, C

N1 - Keywords: Antibodies; Antigen Presentation; Antigens, Bacterial; Antigens, CD80; CD4-Positive T-Lymphocytes; Cell Adhesion Molecules; Cell Count; Cell Line; Child, Preschool; Epithelial Cells; Epithelium; Humans; Infant; Superantigens; T-Lymphocyte Subsets; T-Lymphocytes; Thymus Gland

PY - 1996

Y1 - 1996

N2 - It is generally accepted that thymic epithelial cells (TEC) act as accessory cells in positive selection of pre-T cells. However, our knowledge of the antigen presentation and accessory cell function to human TEC is limited. Here we present results obtained by the use of serum-free cultured human TEC, showing that IFN-gamma-treated TEC are able to support T-cell-mediated responses to the bacterial superantigens (Sag) SEA and SEB, even at very low Sag concentrations. T-cell responses to TEC-presented Sags were dependent on the presence of the adhesion molecules ICAM-1, ICAM-2, LFA-1, and LFA-3, but not on CD4 and CD8 molecules. There is a low but significant expression of B7 molecules on human TEC, and treatment of TEC with anti-B7.1 and anti-B7.2 antibodies before Sag pulsing leads to decreased Sag responses, indicating a significant importance of B7 molecules on TEC. Both CD4+ T-cell lines and CD4+ as well as CD8+ subpopulations of thymocytes showed significant responses, whereas nonseparated thymocytes, CD4+8+, and CD4-CD8- thymocytes did not respond or showed very low responses. In conclusion, the present results demonstrate that cultured human TEC are able to present Sag to thymocytes.

AB - It is generally accepted that thymic epithelial cells (TEC) act as accessory cells in positive selection of pre-T cells. However, our knowledge of the antigen presentation and accessory cell function to human TEC is limited. Here we present results obtained by the use of serum-free cultured human TEC, showing that IFN-gamma-treated TEC are able to support T-cell-mediated responses to the bacterial superantigens (Sag) SEA and SEB, even at very low Sag concentrations. T-cell responses to TEC-presented Sags were dependent on the presence of the adhesion molecules ICAM-1, ICAM-2, LFA-1, and LFA-3, but not on CD4 and CD8 molecules. There is a low but significant expression of B7 molecules on human TEC, and treatment of TEC with anti-B7.1 and anti-B7.2 antibodies before Sag pulsing leads to decreased Sag responses, indicating a significant importance of B7 molecules on TEC. Both CD4+ T-cell lines and CD4+ as well as CD8+ subpopulations of thymocytes showed significant responses, whereas nonseparated thymocytes, CD4+8+, and CD4-CD8- thymocytes did not respond or showed very low responses. In conclusion, the present results demonstrate that cultured human TEC are able to present Sag to thymocytes.

M3 - Journal article

C2 - 9165273

VL - 13

SP - 192

EP - 203

JO - Experimental and Clinical Immunogenetics

JF - Experimental and Clinical Immunogenetics

SN - 0254-9670

IS - 3-4

ER -

ID: 10635938