Forskning ved Københavns Universitet - Københavns Universitet

Forside

Hydrolysis of ATP at only one GyrB subunit is sufficient to promote supercoiling by DNA gyrase

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Mutation of Glu42 to Ala in the B subunit of DNA gyrase abolishes ATP hydrolysis but not nucleotide binding. Gyrase complexes that contain one wild-type and one Ala42 mutant B protein were formed, and the ability of such complexes to hydrolyze ATP was investigated. We found that ATP hydrolysis was able to proceed independently only in the wild-type subunit, albeit at a lower rate. With only one ATP molecule hydrolyzed at a time, gyrase could still perform supercoiling, but the limit of this reaction was lower than that observed when both subunits can hydrolyze the nucleotide.

OriginalsprogEngelsk
TidsskriftThe Journal of Biological Chemistry
Vol/bind273
Udgave nummer41
Sider (fra-til)26305-9
Antal sider5
ISSN0021-9258
StatusUdgivet - 9 okt. 1998

ID: 159085598