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IL-33 induces IL-9 production in human CD4+ T cells and basophils

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Standard

IL-33 induces IL-9 production in human CD4+ T cells and basophils. / Blom, Lars; Poulsen, Britta Cathrina; Jensen, Bettina M.; Hansen, Anker; Poulsen, Lars K.

I: P L o S One, Bind 6, Nr. 7, e21695, 2011.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Blom, L, Poulsen, BC, Jensen, BM, Hansen, A & Poulsen, LK 2011, 'IL-33 induces IL-9 production in human CD4+ T cells and basophils', P L o S One, bind 6, nr. 7, e21695. https://doi.org/10.1371/journal.pone.0021695

APA

Blom, L., Poulsen, B. C., Jensen, B. M., Hansen, A., & Poulsen, L. K. (2011). IL-33 induces IL-9 production in human CD4+ T cells and basophils. P L o S One, 6(7), [e21695]. https://doi.org/10.1371/journal.pone.0021695

Vancouver

Blom L, Poulsen BC, Jensen BM, Hansen A, Poulsen LK. IL-33 induces IL-9 production in human CD4+ T cells and basophils. P L o S One. 2011;6(7). e21695. https://doi.org/10.1371/journal.pone.0021695

Author

Blom, Lars ; Poulsen, Britta Cathrina ; Jensen, Bettina M. ; Hansen, Anker ; Poulsen, Lars K. / IL-33 induces IL-9 production in human CD4+ T cells and basophils. I: P L o S One. 2011 ; Bind 6, Nr. 7.

Bibtex

@article{d9b61b504a1f42ecaa9375133e5598e1,
title = "IL-33 induces IL-9 production in human CD4+ T cells and basophils",
abstract = "IL-33, an IL-1 family member and ligand for the IL-1 receptor-related protein ST2, has been associated with induction of Th2 cytokines such as IL-4, IL-5, and IL-13. Here, we report that IL-33 can initiate IL-9 protein secretion in vitro in human CD4+ T cells and basophils isolated from peripheral blood. TGF-β has been described as a critical factor for IL-9 induction in Th2 cells; however, we found that TGF-β also induces co-production of IL-9 in purified, na{\"i}ve (>99{\%}) CD4(+)CD45RA(+)CD45RO(-)CD25(-) T cells differentiated towards a Th1 profile. Subsequently, it was demonstrated that TGF-β is important, although not an absolute requirement, for IL-9 production in CD4+ T cells. IL-9 production by purified (>95{\%}) human basophils, cultured for 24 h with IL-3 or IL-33, was found, with a strong synergy between the two, likely to be explained by the IL-3 upregulated ST2 expression. Collectively, these data indicate that barrier functioning cells are important for the regulation of IL-9 production by immune cells in inflamed tissue.",
keywords = "Basophils, CD4-Positive T-Lymphocytes, Cell Separation, Gene Expression Regulation, Humans, Interleukin-4, Interleukin-9, Interleukins, RNA, Messenger, Receptors, Cell Surface, Th1 Cells, Th2 Cells, Transforming Growth Factor beta",
author = "Lars Blom and Poulsen, {Britta Cathrina} and Jensen, {Bettina M.} and Anker Hansen and Poulsen, {Lars K.}",
year = "2011",
doi = "10.1371/journal.pone.0021695",
language = "English",
volume = "6",
journal = "P L o S One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "7",

}

RIS

TY - JOUR

T1 - IL-33 induces IL-9 production in human CD4+ T cells and basophils

AU - Blom, Lars

AU - Poulsen, Britta Cathrina

AU - Jensen, Bettina M.

AU - Hansen, Anker

AU - Poulsen, Lars K.

PY - 2011

Y1 - 2011

N2 - IL-33, an IL-1 family member and ligand for the IL-1 receptor-related protein ST2, has been associated with induction of Th2 cytokines such as IL-4, IL-5, and IL-13. Here, we report that IL-33 can initiate IL-9 protein secretion in vitro in human CD4+ T cells and basophils isolated from peripheral blood. TGF-β has been described as a critical factor for IL-9 induction in Th2 cells; however, we found that TGF-β also induces co-production of IL-9 in purified, naïve (>99%) CD4(+)CD45RA(+)CD45RO(-)CD25(-) T cells differentiated towards a Th1 profile. Subsequently, it was demonstrated that TGF-β is important, although not an absolute requirement, for IL-9 production in CD4+ T cells. IL-9 production by purified (>95%) human basophils, cultured for 24 h with IL-3 or IL-33, was found, with a strong synergy between the two, likely to be explained by the IL-3 upregulated ST2 expression. Collectively, these data indicate that barrier functioning cells are important for the regulation of IL-9 production by immune cells in inflamed tissue.

AB - IL-33, an IL-1 family member and ligand for the IL-1 receptor-related protein ST2, has been associated with induction of Th2 cytokines such as IL-4, IL-5, and IL-13. Here, we report that IL-33 can initiate IL-9 protein secretion in vitro in human CD4+ T cells and basophils isolated from peripheral blood. TGF-β has been described as a critical factor for IL-9 induction in Th2 cells; however, we found that TGF-β also induces co-production of IL-9 in purified, naïve (>99%) CD4(+)CD45RA(+)CD45RO(-)CD25(-) T cells differentiated towards a Th1 profile. Subsequently, it was demonstrated that TGF-β is important, although not an absolute requirement, for IL-9 production in CD4+ T cells. IL-9 production by purified (>95%) human basophils, cultured for 24 h with IL-3 or IL-33, was found, with a strong synergy between the two, likely to be explained by the IL-3 upregulated ST2 expression. Collectively, these data indicate that barrier functioning cells are important for the regulation of IL-9 production by immune cells in inflamed tissue.

KW - Basophils

KW - CD4-Positive T-Lymphocytes

KW - Cell Separation

KW - Gene Expression Regulation

KW - Humans

KW - Interleukin-4

KW - Interleukin-9

KW - Interleukins

KW - RNA, Messenger

KW - Receptors, Cell Surface

KW - Th1 Cells

KW - Th2 Cells

KW - Transforming Growth Factor beta

U2 - 10.1371/journal.pone.0021695

DO - 10.1371/journal.pone.0021695

M3 - Journal article

C2 - 21765905

VL - 6

JO - P L o S One

JF - P L o S One

SN - 1932-6203

IS - 7

M1 - e21695

ER -

ID: 33729868