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Imaging N-acyl homoserine lactone quorum sensing in vivo

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E. coli monitor strain in the lung tissues, indicating production and excretion of AHLs in vivo by the infecting P. aeruginosa.

OriginalsprogEngelsk
TitelQuorum Sensing : Methods and Protocols
RedaktørerLivia Leoni, Giordano Rampion
Antal sider10
Vol/bind1673
ForlagHumana Press
Publikationsdato2018
Sider203-212
ISBN (Trykt)978-1-4939-7308-8
ISBN (Elektronisk)978-1-4939-7309-5
DOI
StatusUdgivet - 2018
NavnMethods in Molecular Biology
Nummer1673
ISSN1064-3745

ID: 186872679