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Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C

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Standard

Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C. / Jensen, T J; Kharazmi, A; Shand, G; Nielsen, H; Tvede, M.

I: Acta Pathologica Microbiologica et Immunologica Scandinavica, Bind 104, Nr. 1, 1996, s. 54-60.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Jensen, TJ, Kharazmi, A, Shand, G, Nielsen, H & Tvede, M 1996, 'Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C', Acta Pathologica Microbiologica et Immunologica Scandinavica, bind 104, nr. 1, s. 54-60.

APA

Jensen, T. J., Kharazmi, A., Shand, G., Nielsen, H., & Tvede, M. (1996). Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C. Acta Pathologica Microbiologica et Immunologica Scandinavica, 104(1), 54-60.

Vancouver

Jensen TJ, Kharazmi A, Shand G, Nielsen H, Tvede M. Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C. Acta Pathologica Microbiologica et Immunologica Scandinavica. 1996;104(1):54-60.

Author

Jensen, T J ; Kharazmi, A ; Shand, G ; Nielsen, H ; Tvede, M. / Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C. I: Acta Pathologica Microbiologica et Immunologica Scandinavica. 1996 ; Bind 104, Nr. 1. s. 54-60.

Bibtex

@article{28cdf1401c9e11df8ed1000ea68e967b,
title = "Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C",
abstract = "The aim of the study was to measure and compare the oxidative burst, chemotaxis and cytokine production of human white blood cells, stimulated with meningococcal lipopolysaccharides (LPS) extracted from three different serogroups (A, B and C) of Neisseria meningitidis, and to evaluate whether convalescent sera from patients with meningococcal disease could modify cell stimulation of LPS. All three preparations of LPS from groups A, B and C were tested using the Limulus amoebocyte lysate assay (LAL), and the KDO concentrations of the LPS extracts were measured. Equivalent amounts of biologically active LPS, judged by LAL, and LPS with the same KDO concentration were assayed. IL-1alpha, IL-1beta, IL-6 and TNF-alpha production was stimulated by all three LPS preparations. All three preparations stimulated oxidative burst in monocytes (MNC). Only group A LPS stimulated neutrophil chemotaxis, while none of the three LPS stimulated superoxide production. Pooled convalescent sera from five patients with meningococcal disease suppressed the activity of neutrophils stimulated with LPS from groups B and C (p<0.05, Mann-Whitney U-test).",
author = "Jensen, {T J} and A Kharazmi and G Shand and H Nielsen and M Tvede",
note = "Keywords: Cells, Cultured; Chemotaxis; Cytokines; Humans; Leukocytes, Mononuclear; Lipopolysaccharides; Neisseria meningitidis; Respiratory Burst",
year = "1996",
language = "English",
volume = "104",
pages = "54--60",
journal = "A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica",
issn = "0903-4641",
publisher = "Wiley Online",
number = "1",

}

RIS

TY - JOUR

T1 - Immunological properties of meningococcal lipopolysaccharide from serogroups A, B & C

AU - Jensen, T J

AU - Kharazmi, A

AU - Shand, G

AU - Nielsen, H

AU - Tvede, M

N1 - Keywords: Cells, Cultured; Chemotaxis; Cytokines; Humans; Leukocytes, Mononuclear; Lipopolysaccharides; Neisseria meningitidis; Respiratory Burst

PY - 1996

Y1 - 1996

N2 - The aim of the study was to measure and compare the oxidative burst, chemotaxis and cytokine production of human white blood cells, stimulated with meningococcal lipopolysaccharides (LPS) extracted from three different serogroups (A, B and C) of Neisseria meningitidis, and to evaluate whether convalescent sera from patients with meningococcal disease could modify cell stimulation of LPS. All three preparations of LPS from groups A, B and C were tested using the Limulus amoebocyte lysate assay (LAL), and the KDO concentrations of the LPS extracts were measured. Equivalent amounts of biologically active LPS, judged by LAL, and LPS with the same KDO concentration were assayed. IL-1alpha, IL-1beta, IL-6 and TNF-alpha production was stimulated by all three LPS preparations. All three preparations stimulated oxidative burst in monocytes (MNC). Only group A LPS stimulated neutrophil chemotaxis, while none of the three LPS stimulated superoxide production. Pooled convalescent sera from five patients with meningococcal disease suppressed the activity of neutrophils stimulated with LPS from groups B and C (p<0.05, Mann-Whitney U-test).

AB - The aim of the study was to measure and compare the oxidative burst, chemotaxis and cytokine production of human white blood cells, stimulated with meningococcal lipopolysaccharides (LPS) extracted from three different serogroups (A, B and C) of Neisseria meningitidis, and to evaluate whether convalescent sera from patients with meningococcal disease could modify cell stimulation of LPS. All three preparations of LPS from groups A, B and C were tested using the Limulus amoebocyte lysate assay (LAL), and the KDO concentrations of the LPS extracts were measured. Equivalent amounts of biologically active LPS, judged by LAL, and LPS with the same KDO concentration were assayed. IL-1alpha, IL-1beta, IL-6 and TNF-alpha production was stimulated by all three LPS preparations. All three preparations stimulated oxidative burst in monocytes (MNC). Only group A LPS stimulated neutrophil chemotaxis, while none of the three LPS stimulated superoxide production. Pooled convalescent sera from five patients with meningococcal disease suppressed the activity of neutrophils stimulated with LPS from groups B and C (p<0.05, Mann-Whitney U-test).

M3 - Journal article

C2 - 8645459

VL - 104

SP - 54

EP - 60

JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica

SN - 0903-4641

IS - 1

ER -

ID: 18080942