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In vitro characterization of the Ac locus in white clover (Trifolium repens L.)

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Microsomal fractions from developing shoots of adult white clover plants (of genotype Ac Ac) and cotyledons of dark germinated clover seedlings can synthesize 2-hydroxy-2-methylpropanenitrile and 2-hydroxy-2-methylbutanenitrile, the aglycone precursors of the cyanogenic glucosides, linamarin and lotaustralin, from various precursors in the presence of NADPH. l-Valine, 2-methylpropanal oxime, and 2-methylpropanenitrile are converted to 2-hydroxy-2-methylpropanenitrile and are detected as cyanide and acetone. l-Isoleucine and 2-methylbutanal oxime are converted to 2-hydroxy-2-methylbutanenitrile and are detected as cyanide and 2-butanone. At least two steps in these conversions are missing in microsomes from plants of genotype ac ac.

OriginalsprogEngelsk
TidsskriftArchives of Biochemistry and Biophysics
Vol/bind218
Udgave nummer1
Sider (fra-til)38-45
Antal sider8
ISSN0003-9861
DOI
StatusUdgivet - 1 okt. 1982

ID: 201511980