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In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos

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Standard

In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos. / Gui, Tao; Zhang, Meiling; Chen, Jianwen; Zhang, Yuanliang; Zhou, Naru; Zhang, Yu; Tao, Jia; Sui, Liucai; Li, Yunsheng; Liu, Ya; Zhang, Xiaorong; Zhang, Yunhai.

I: Biotechnology Letters, Bind 34, Nr. 8, 2012, s. 1445-1452.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Gui, T, Zhang, M, Chen, J, Zhang, Y, Zhou, N, Zhang, Y, Tao, J, Sui, L, Li, Y, Liu, Y, Zhang, X & Zhang, Y 2012, 'In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos', Biotechnology Letters, bind 34, nr. 8, s. 1445-1452. https://doi.org/10.1007/s10529-012-0930-7

APA

Gui, T., Zhang, M., Chen, J., Zhang, Y., Zhou, N., Zhang, Y., ... Zhang, Y. (2012). In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos. Biotechnology Letters, 34(8), 1445-1452. https://doi.org/10.1007/s10529-012-0930-7

Vancouver

Gui T, Zhang M, Chen J, Zhang Y, Zhou N, Zhang Y o.a. In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos. Biotechnology Letters. 2012;34(8):1445-1452. https://doi.org/10.1007/s10529-012-0930-7

Author

Gui, Tao ; Zhang, Meiling ; Chen, Jianwen ; Zhang, Yuanliang ; Zhou, Naru ; Zhang, Yu ; Tao, Jia ; Sui, Liucai ; Li, Yunsheng ; Liu, Ya ; Zhang, Xiaorong ; Zhang, Yunhai. / In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos. I: Biotechnology Letters. 2012 ; Bind 34, Nr. 8. s. 1445-1452.

Bibtex

@article{5cec15913eda4449b55dd26113f611d5,
title = "In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos",
abstract = "A vector expressing human lysozyme (pBC1-hLYZ-GFP-Neo) was evaluated for gene and protein expression following liposome-mediated transformation of C-127 mouse mammary cancer cells. Cultures of G418-resistant clones were harvested 24-72 h after induction with prolactin, insulin and hydrocortisone. Target gene expression was analyzed by RT-PCR and Western blot and recombinant human lysozyme (rhLYZ) bacteriostatic activity was also evaluated. The hLYZ gene was correctly transcribed and translated in C-127 cells and hLYZ inhibited gram-positive bacterial growth, indicating the potential of this expression vector for development of a mammary gland bioreactor in goats. Guanzhong dairy goat skin fibroblasts transfected with pBC1-hLYZ-GFP-Neo were used to construct a goat embryo transgenically expressing rhLYZ by somatic nuclear transplantation with a blastocyst rate of 9.0 ± 2.8 {\%}. These data establish the basis for cultivation of mastitis-resistant hLYZ transgenic goats.",
keywords = "Animals, Animals, Genetically Modified, Bioreactors, Cell Line, Tumor, Embryo, Mammalian, Female, Genetic Vectors, Goats, Green Fluorescent Proteins, Humans, Male, Mammary Glands, Animal, Mice, Muramidase, Polymerase Chain Reaction, Recombinant Proteins",
author = "Tao Gui and Meiling Zhang and Jianwen Chen and Yuanliang Zhang and Naru Zhou and Yu Zhang and Jia Tao and Liucai Sui and Yunsheng Li and Ya Liu and Xiaorong Zhang and Yunhai Zhang",
year = "2012",
doi = "10.1007/s10529-012-0930-7",
language = "English",
volume = "34",
pages = "1445--1452",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer",
number = "8",

}

RIS

TY - JOUR

T1 - In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos

AU - Gui, Tao

AU - Zhang, Meiling

AU - Chen, Jianwen

AU - Zhang, Yuanliang

AU - Zhou, Naru

AU - Zhang, Yu

AU - Tao, Jia

AU - Sui, Liucai

AU - Li, Yunsheng

AU - Liu, Ya

AU - Zhang, Xiaorong

AU - Zhang, Yunhai

PY - 2012

Y1 - 2012

N2 - A vector expressing human lysozyme (pBC1-hLYZ-GFP-Neo) was evaluated for gene and protein expression following liposome-mediated transformation of C-127 mouse mammary cancer cells. Cultures of G418-resistant clones were harvested 24-72 h after induction with prolactin, insulin and hydrocortisone. Target gene expression was analyzed by RT-PCR and Western blot and recombinant human lysozyme (rhLYZ) bacteriostatic activity was also evaluated. The hLYZ gene was correctly transcribed and translated in C-127 cells and hLYZ inhibited gram-positive bacterial growth, indicating the potential of this expression vector for development of a mammary gland bioreactor in goats. Guanzhong dairy goat skin fibroblasts transfected with pBC1-hLYZ-GFP-Neo were used to construct a goat embryo transgenically expressing rhLYZ by somatic nuclear transplantation with a blastocyst rate of 9.0 ± 2.8 %. These data establish the basis for cultivation of mastitis-resistant hLYZ transgenic goats.

AB - A vector expressing human lysozyme (pBC1-hLYZ-GFP-Neo) was evaluated for gene and protein expression following liposome-mediated transformation of C-127 mouse mammary cancer cells. Cultures of G418-resistant clones were harvested 24-72 h after induction with prolactin, insulin and hydrocortisone. Target gene expression was analyzed by RT-PCR and Western blot and recombinant human lysozyme (rhLYZ) bacteriostatic activity was also evaluated. The hLYZ gene was correctly transcribed and translated in C-127 cells and hLYZ inhibited gram-positive bacterial growth, indicating the potential of this expression vector for development of a mammary gland bioreactor in goats. Guanzhong dairy goat skin fibroblasts transfected with pBC1-hLYZ-GFP-Neo were used to construct a goat embryo transgenically expressing rhLYZ by somatic nuclear transplantation with a blastocyst rate of 9.0 ± 2.8 %. These data establish the basis for cultivation of mastitis-resistant hLYZ transgenic goats.

KW - Animals

KW - Animals, Genetically Modified

KW - Bioreactors

KW - Cell Line, Tumor

KW - Embryo, Mammalian

KW - Female

KW - Genetic Vectors

KW - Goats

KW - Green Fluorescent Proteins

KW - Humans

KW - Male

KW - Mammary Glands, Animal

KW - Mice

KW - Muramidase

KW - Polymerase Chain Reaction

KW - Recombinant Proteins

U2 - 10.1007/s10529-012-0930-7

DO - 10.1007/s10529-012-0930-7

M3 - Journal article

C2 - 22526423

VL - 34

SP - 1445

EP - 1452

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 8

ER -

ID: 135279778