Forskning ved Københavns Universitet - Københavns Universitet

Forside

In vitro metabolism and permeation studies in rat jejunum: organic chromium compared to inorganic chromium

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

The purpose of these studies was to compare the in vitro absorption of two inorganic chromium(III) compounds: chromium chloride and chromium nitrate, with organic chromium(III)-picolinate; and to investigate if any in vitro metabolism of chromium(VI) takes place. The in vitro metabolism studies showed that chromium (VI) was reduced by artificial gastric juice. The reduction followed first order kinetics with a half-life of 23 min. The studies also showed that the chromium picolinate complex was stable in artificial gastric juice for 4 hours. By the rat everted gut sac technique, chromium chloride, chromium nitrate and chromium picolinate penetrated the rat jejunum with 165 +/- 59, 160 +/- 26 and 127 +/- 36 ng chromium per g rat jejunum, respectively, whereas the permeability coefficients (Papp) were 0.7 +/- 0.3, 1.0 +/- 0.4, and 9.6 +/- 2.2 microns/min, respectively. Absorption studies on pig intestine in Ussing chambers showed a nearly total adsorption of chromium(III) by the chambers, resulting in unreliable data.
OriginalsprogEngelsk
TidsskriftJournal of Trace Elements in Medicine and Biology
Vol/bind13
Udgave nummer1-2
Sider (fra-til)82-8
Antal sider7
ISSN0946-672X
DOI
StatusUdgivet - 1999

ID: 44053761