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Increased DNA binding and sequence discrimination of PNA oligomers containing 2,6-diaminopurine

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The synthesis of a diaminopurine PNA monomer, N-[N6-(benzyloxycarbonyl)-2,6-diaminopurine-9-yl] acetyl-N-(2-t-butyloxycarbonylaminoethyl)glycine, and the incorporation of this monomer into PNA oligomers are described. Substitution of adenine by diaminopurine in PNA oligomers increased the T m of duplexes formed with complementary DNA, RNA or PNA by 2.5-6.5 degrees C per diaminopurine. Furthermore, discrimination against mismatches facing the diaminopurine in the hybridizing oligomer is improved. Finally, a homopurine decamer PNA containing six diaminopurines is shown to form a (gel shift) stable strand displacement complex with a target in a 246 bp double-stranded DNA fragment.

OriginalsprogEngelsk
TidsskriftNucleic Acids Research
Vol/bind25
Udgave nummer22
Sider (fra-til)4639-43
Antal sider5
ISSN0305-1048
StatusUdgivet - 15 nov. 1997

ID: 203632904