Forskning ved Københavns Universitet - Københavns Universitet

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Indirect imaging of cardiac-specific transgene expression using a bidirectional two-step transcriptional amplification strategy

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

  • I Y Chen
  • O Gheysens
  • S Ray
  • Q Wang
  • P Padmanabhan
  • R Paulmurugan
  • A M Loening
  • M Rodriguez-Porcel
  • J K Willmann
  • A Y Sheikh
  • Nielsen, Carsten Haagen
  • G Hoyt
  • C H Contag
  • R C Robbins
  • S Biswal
  • J C Wu
  • S S Gambhir
Transcriptional targeting for cardiac gene therapy is limited by the relatively weak activity of most cardiac-specific promoters. We have developed a bidirectional plasmid vector, which uses a two-step transcriptional amplification (TSTA) strategy to enhance the expression of two optical reporter genes, firefly luciferase (fluc) and Renilla luciferase (hrluc), driven by the cardiac troponin T (cTnT) promoter. The vector was characterized in vitro and in living mice using luminometry and bioluminescence imaging to assess its ability to mediate strong, correlated reporter gene expression in a cardiac cell line and the myocardium, while minimizing expression in non-cardiac cell lines and the liver. In vitro, the TSTA system significantly enhanced cTnT-mediated reporter gene expression with moderate preservation of cardiac specificity. After intramyocardial and hydrodynamic tail vein delivery of an hrluc-enhanced variant of the vector, long-term fluc expression was observed in the heart, but not in the liver. In both the cardiac cell line and the myocardium, fluc expression correlated well with hrluc expression. These results show the vector's ability to effectively amplify and couple transgene expression in a cardiac-specific manner. Further replacement of either reporter gene with a therapeutic gene should allow non-invasive imaging of targeted gene therapy in living subjects.
OriginalsprogEngelsk
TidsskriftGene Therapy (Basingstoke)
Vol/bind17
Udgave nummer7
Sider (fra-til)827-38
Antal sider12
ISSN0969-7128
DOI
StatusUdgivet - 2010

ID: 32982715