Forskning ved Københavns Universitet - Københavns Universitet

Forside

Inhibition of yeast growth during long term exposure to laser light around 1064 nm

Publikation: Bidrag til tidsskriftKonferenceartikelForskningfagfællebedømt

We have studied the effect of a 1070 nm continuous wave Ytterbium fiber laser on exponentially growing Saccharomyces cerevisiae yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered yeast cells over a span of 4 hours. The cells were immobilized onto Concanavalin A covered microscope slides and the growth was measured using the area increase of the cells in 2D. Using a continuous dual beam plane wave with a uniform spatial intensity distribution, we found that a continuous radiant flux through a single cell as low as 0.5 mW in 1.5 hours significantly changed the growth and division rate of S. cerevisiae. With the dual beam setup used we were able to successfully manipulate single S. cerevisiae cells in 3 dimensions with a minimum flux thorough the cell of 3.5 mW. In the regime investigated from 0.7 mW to 2.6 mW we found no threshold for the photo damage, but rather a continuous response to the increased accumulated dose.

 

OriginalsprogEngelsk
TidsskriftProceedings of SPIE, the International Society for Optical Engineering
Antal sider7
ISSN0277-786X
DOI
StatusUdgivet - 2009
BegivenhedComplex Light and Optical Forces - San Jose, CA, USA
Varighed: 28 jan. 2009 → …
Konferencens nummer: 3

Konference

KonferenceComplex Light and Optical Forces
Nummer3
LandUSA
BySan Jose, CA
Periode28/01/2009 → …

Bibliografisk note

Volumne: 7227

ID: 18651875