Forskning ved Københavns Universitet - Københavns Universitet

Forside

Insulin action in human thighs after one-legged immobilization

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Insulin action was assessed in thighs of five healthy young males who had one knee immobilized for 7 days by a splint. The splint was not worn in bed. Subjects also used crutches to prevent weight bearing of the immobilized leg. Immobilization decreased the activity of citrate synthase and 3-OH-acyl-CoA-dehydrogenase in the vastus lateralis muscle by 9 and 14%, respectively, and thigh volume by 5%. After 7 days of immobilization, a two-step euglycemic hyperinsulinemic clamp procedure combined with arterial and bilateral femoral venous catheterization was performed. Insulin action on glucose uptake and tyrosine release of the thighs at mean plasma insulin concentrations of 67 (clamp step I) and 447 microU/ml (clamp step II) was decreased by immobilization, whereas immobilization did not affect insulin action on thigh exchange of free fatty acids, glycerol, O2, or potassium. Before and during the clamp step I, lactate release was significantly higher in the immobilized than in the control thigh. Seven days of one-legged immobilization causes local decreased insulin action on thigh glucose uptake and net protein degradation.

OriginalsprogEngelsk
TidsskriftJournal of Applied Physiology
Vol/bind67
Udgave nummer1
Sider (fra-til)19-23
Antal sider5
ISSN8750-7587
StatusUdgivet - 1989

ID: 154756678